通过二甲基亚砜梯度沉降法测定仙台病毒RNA的分子量
Molecular weight determination of Sendai RNA by dimethyl sulfoxide gradient sedimentation.
作者信息
Kolakofsky D, Bruschi A
出版信息
J Virol. 1973 May;11(5):615-20. doi: 10.1128/JVI.11.5.615-620.1973.
Abstract
The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 x 10(6). Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 x 10(6) is considerably smaller than the estimates of 6 x 10(6) to 7 x 10(6) determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.
摘要
通过在含有99%二甲基亚砜（DMSO）的蔗糖梯度中进行沉降分析，已确定仙台病毒大RNA的分子量为2.3×10⁶。从99%DMSO中回收的仙台RNA在普通蔗糖梯度中于46至48秒处与未变性的仙台RNA共同沉降。2.3×10⁶的分子量值明显小于在非变性条件下确定的6×10⁶至7×10⁶的估计值，这表明仙台RNA具有独特的结构。
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