通过核酸酶消化法测定动物RNA病毒基因组的分子量。I. 水疱性口炎病毒及其缺陷型T颗粒
Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle.
作者信息
Repik P, Bishop D H
出版信息
J Virol. 1973 Nov;12(5):969-83. doi: 10.1128/JVI.12.5.969-983.1973.
Abstract
A procedure has been developed for the determination of the weight of animal RNA virus genomes using controlled nuclease digestions and computation of the moles of oligonucleotides obtained from 1 mol of RNA. Using both pancreatic RNase and RNase T(1) to digest viral RNA labeled by (3)H-uridine, (3)H-cytidine, or (3)H-guanosine, the weight of the virion RNA of vesicular stomatitis virus (VSV) is estimated as 3.82 +/- 0.14 x 10(6) whereas that of the VSV-defective T particle is estimated as 1.23 +/- 0.04 x 10(6).
摘要
已开发出一种程序，用于通过可控核酸酶消化以及计算从1摩尔RNA获得的寡核苷酸摩尔数来测定动物RNA病毒基因组的重量。使用胰核糖核酸酶和核糖核酸酶T(1)消化用(3)H-尿苷、(3)H-胞苷或(3)H-鸟苷标记的病毒RNA，估计水疱性口炎病毒(VSV)的病毒体RNA重量为3.82 +/- 0.14 x 10(6)，而VSV缺陷型T颗粒的重量估计为1.23 +/- 0.04 x 10(6)。
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