[高强度激光照射后原代培养的鸡成肌细胞和神经母细胞的肌酸激酶活性]
[The creatine kinase activity of chick myoblasts and neuroblasts in primary culture after intensive laser irradiation].
作者信息
Matiushichev V B, Sokolov I N, Soldatov A I, Taame N A
出版信息
Tsitologiia. 1995;37(9-10):905-9.
The influence of green-yellow light of copper laser (mean power of impulse-periodic emanation 3 W, working density of power in experiment is 200 mW/cm2) on biochemical indices of chick embryo myoblasts and neuroblasts in primary culture was studied. The content of water extracted protein was found to increase progressively, the total and specific activities of creatine kinase elevated considerably in both the cell cultures under irradiation with the total doses equal to 3 and 15 J. However, with dose of 30 J these parameters corresponded to the level characteristic of nonirradiated cultures. Mechanisms of stimulating action of highly intensive laser emanation on cell functions are discussed.
研究了铜激光的绿黄色光（脉冲-周期发射的平均功率为3W，实验中的工作功率密度为200mW/cm²）对原代培养的鸡胚成肌细胞和神经母细胞生化指标的影响。发现水提取蛋白含量逐渐增加，在总剂量等于3J和15J的照射下，两种细胞培养物中肌酸激酶的总活性和比活性均显著升高。然而，在30J的剂量下，这些参数与未照射培养物的特征水平相当。讨论了高强度激光发射对细胞功能的刺激作用机制。
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