原胶原蛋白的无细胞合成：以L-929成纤维细胞作为皮肤松弛症的细胞模型
Cell-free synthesis of procollagen: L-929 fibroblasts as a cellular model for dermatosparaxis.
作者信息
Kerwar S S, Cardinale G J, Kohn L D, Spears C L, Stassen F L
出版信息
Proc Natl Acad Sci U S A. 1973 May;70(5):1378-82. doi: 10.1073/pnas.70.5.1378.
Abstract
A cell-free system that actively synthesizes collagen was prepared from L-929 fibroblasts. Chromatographic and electrophoretic techniques were used to demonstrate that the only collagenous products are pro alpha(1) and pro alpha(2) chains. The collagen synthesized by the cell-free system was also compared to the collagen extracted from the cells. The cellular collagen was composed of aggregates of pro-alpha chains, while no alpha chains were found. Procollagen peptidase activity could not be detected in the cells, and the activity present in the medium was low, comparable to that in dermatosparaxic cell cultures. These properties indicate that L-929 cells may be a model system for dermatosparaxis.
摘要
从L - 929成纤维细胞制备了一种能积极合成胶原蛋白的无细胞系统。采用色谱和电泳技术证明，唯一的胶原产物是前α(1)链和前α(2)链。还将无细胞系统合成的胶原蛋白与从细胞中提取的胶原蛋白进行了比较。细胞胶原蛋白由前α链聚集体组成，未发现α链。在细胞中未检测到前胶原肽酶活性，培养基中的活性较低，与皮肤松弛症细胞培养中的活性相当。这些特性表明L - 929细胞可能是皮肤松弛症的一个模型系统。
相似文献
1
Cell-free synthesis of procollagen: L-929 fibroblasts as a cellular model for dermatosparaxis.原胶原蛋白的无细胞合成：以L-929成纤维细胞作为皮肤松弛症的细胞模型
Proc Natl Acad Sci U S A. 1973 May;70(5):1378-82. doi: 10.1073/pnas.70.5.1378.
2
Collagen synthesis on polyribosomes of cultured mammalian fibroblasts.
J Mol Biol. 1967 May 28;26(1):1-18. doi: 10.1016/0022-2836(67)90257-4.
3
Secretion and extracellular processing of procollagen by cultured human fibroblasts.培养的人成纤维细胞对前胶原的分泌及细胞外加工
Proc Natl Acad Sci U S A. 1973 Feb;70(2):361-5. doi: 10.1073/pnas.70.2.361.
4
Collagen synthesis by lathyrogen-treated 3T6 fibroblasts.
Connect Tissue Res. 1974;2(2):91-3. doi: 10.3109/03008207409152093.
5
Identification of two distinct species of procollagen synthesized by a clonal line of calf dermatosparactic cells.从小牛皮肤松弛症细胞克隆系中鉴定出两种不同的前胶原。
Nat New Biol. 1973 Aug 8;244(136):188-90. doi: 10.1038/newbio244188a0.
6
Production of procollagen by human fibroblasts in culture.培养的人成纤维细胞中前胶原的产生。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3260-2. doi: 10.1073/pnas.69.11.3260.
7
Evidence for a relationship between Ehlers-Danlos type VII C in humans and bovine dermatosparaxis.人类埃勒斯-当洛综合征VII C型与牛皮肤松弛症之间关系的证据。
Nat Genet. 1992 Jun;1(3):214-7. doi: 10.1038/ng0692-214.
8
Precursors of collagen secreted by cultured human fibroblasts.培养的人成纤维细胞分泌的胶原蛋白前体。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3655-9. doi: 10.1073/pnas.69.12.3655.
9
NH2-terminal extensions on skin collagen from sheep with a genetic defect in conversion of procollagen into collagen.患有前胶原转化为胶原基因缺陷的绵羊皮肤胶原蛋白的氨基末端延伸。
Biochemistry. 1976 Jun 29;15(13):2853-62. doi: 10.1021/bi00658a024.
10
The synthesis of underhydroxylated collagen by 3 T6 mouse fibroblasts in culture.3T6小鼠成纤维细胞在培养中合成低羟基化胶原蛋白。
Biochim Biophys Acta. 1972 Apr 15;263(2):397-405. doi: 10.1016/0005-2795(72)90091-8.
引用本文的文献
1
Visualized procollagen Iα1 demonstrates the intracellular processing of propeptides.可视化前胶原 Iα1 显示了前肽的细胞内加工过程。
Life Sci Alliance. 2022 Feb 18;5(5). doi: 10.26508/lsa.202101060. Print 2022 May.
2
Coalignment of plasma membrane channels and protrusions (fibripositors) specifies the parallelism of tendon.质膜通道与突起（纤维定位器）的共排列决定了肌腱的平行度。
J Cell Biol. 2004 May 24;165(4):553-63. doi: 10.1083/jcb.200312071.
3
The identification of collagen messenger RNA.胶原蛋白信使核糖核酸的鉴定。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4208-12. doi: 10.1073/pnas.71.10.4208.
4
Collagen metabolism in cultured osteoblasts from osteogenesis imperfecta patients.成骨不全症患者培养成骨细胞中的胶原蛋白代谢
Biochem J. 1992 Aug 15;286 ( Pt 1)(Pt 1):73-7. doi: 10.1042/bj2860073.
5
Characterization of the synthesized collagen by mouse L-929 (L-5) cells.通过小鼠L-929（L-5）细胞对合成胶原蛋白进行表征。
Arch Dermatol Res (1975). 1977 Oct 27;260(1):7-15. doi: 10.1007/BF00558009.
6
Effects of glucocorticosteroids on primary human skin fibroblasts. I. Inhibition of the proliferation of cultured primary human skin and mouse L929 fibroblasts.糖皮质激素对原代人皮肤成纤维细胞的影响。I. 对培养的原代人皮肤成纤维细胞和小鼠L929成纤维细胞增殖的抑制作用。
Arch Dermatol Res (1975). 1977 Aug 22;259(2):117-23. doi: 10.1007/BF00557951.
7
Translation of embryonic-chick tendon procollagen messenger ribonucleic acid in two cell-free protein-synthesizing systems.在两个无细胞蛋白质合成系统中对鸡胚胎肌腱前胶原信使核糖核酸的翻译
Biochem J. 1979 Jul 15;182(1):81-93. doi: 10.1042/bj1820081.
本文引用的文献
1
Conversion of proline-C14 to peptide-bound hydroxyproline-C14 in a cell-free system from chick embryo.在鸡胚无细胞体系中脯氨酸-C14 向肽结合羟脯氨酸-C14 的转化
Biochem Biophys Res Commun. 1961 Nov 20;6:184-90. doi: 10.1016/0006-291x(61)90126-7.
2
Collagen chain formation and peptidyl proline hydroxylation in monolayer tissue cultures of L-929 fibroblasts.L-929成纤维细胞单层组织培养中的胶原蛋白链形成及肽基脯氨酸羟化作用
Arch Biochem Biophys. 1969 Jan;129(1):308-16. doi: 10.1016/0003-9861(69)90180-5.
3
Isolation of a protein fraction from reticulocyte ribosomes required for de novo synthesis of hemoglobin.从网织红细胞核糖体中分离出血红蛋白从头合成所需的蛋白质组分。
Arch Biochem Biophys. 1968 May;125(2):632-46. doi: 10.1016/0003-9861(68)90622-x.
4
A convenient method of preparing polyacrylamide gels for liquid scintillation spectrometry.一种制备用于液体闪烁光谱分析的聚丙烯酰胺凝胶的简便方法。
Anal Biochem. 1968 Jan;22(1):89-98. doi: 10.1016/0003-2697(68)90262-5.
5
Purification and properties of collagen proline hydroxylase from newborn rat skin.新生大鼠皮肤中胶原蛋白脯氨酸羟化酶的纯化及性质
Arch Biochem Biophys. 1970 Aug;139(2):329-39. doi: 10.1016/0003-9861(70)90485-6.
6
A collagenous tissue dysplasia of calves.犊牛的一种胶原组织发育异常。
Lab Invest. 1970 Sep;23(3):307-14.
7
Procollagen peptidase: an enzyme excising the coordination peptides of procollagen.前胶原肽酶：一种切除前胶原中协调肽的酶。
Proc Natl Acad Sci U S A. 1971 Dec;68(12):3054-8. doi: 10.1073/pnas.68.12.3054.
8
Collagen synthesis on polysomes in vivo and in vitro.体内和体外多核糖体上的胶原蛋白合成
Nat New Biol. 1971 Jul 14;232(28):37-40. doi: 10.1038/newbio232037a0.
9
The nature of the collagen synthesized by cultured human fibroblasts.培养的人成纤维细胞合成的胶原蛋白的性质。
Proc Natl Acad Sci U S A. 1971 Feb;68(2):454-8. doi: 10.1073/pnas.68.2.454.
10
Collagen made of extended -chains, procollagen, in genetically-defective dermatosparaxic calves.基因缺陷的皮肤松垂症小牛中由伸展链组成的胶原蛋白，即前胶原。
Eur J Biochem. 1971 Dec 10;23(3):533-43. doi: 10.1111/j.1432-1033.1971.tb01651.x.
Zotero 插件