Kerwar S S, Cardinale G J, Kohn L D, Spears C L, Stassen F L
Proc Natl Acad Sci U S A. 1973 May;70(5):1378-82. doi: 10.1073/pnas.70.5.1378.
A cell-free system that actively synthesizes collagen was prepared from L-929 fibroblasts. Chromatographic and electrophoretic techniques were used to demonstrate that the only collagenous products are pro alpha(1) and pro alpha(2) chains. The collagen synthesized by the cell-free system was also compared to the collagen extracted from the cells. The cellular collagen was composed of aggregates of pro-alpha chains, while no alpha chains were found. Procollagen peptidase activity could not be detected in the cells, and the activity present in the medium was low, comparable to that in dermatosparaxic cell cultures. These properties indicate that L-929 cells may be a model system for dermatosparaxis.
从L - 929成纤维细胞制备了一种能积极合成胶原蛋白的无细胞系统。采用色谱和电泳技术证明，唯一的胶原产物是前α(1)链和前α(2)链。还将无细胞系统合成的胶原蛋白与从细胞中提取的胶原蛋白进行了比较。细胞胶原蛋白由前α链聚集体组成，未发现α链。在细胞中未检测到前胶原肽酶活性，培养基中的活性较低，与皮肤松弛症细胞培养中的活性相当。这些特性表明L - 929细胞可能是皮肤松弛症的一个模型系统。
