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1
Production of procollagen by human fibroblasts in culture.培养的人成纤维细胞中前胶原的产生。
Proc Natl Acad Sci U S A. 1972 Nov;69(11):3260-2. doi: 10.1073/pnas.69.11.3260.
2
Precursors of collagen secreted by cultured human fibroblasts.培养的人成纤维细胞分泌的胶原蛋白前体。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3655-9. doi: 10.1073/pnas.69.12.3655.
3
Secretion and extracellular processing of procollagen by cultured human fibroblasts.培养的人成纤维细胞对前胶原的分泌及细胞外加工
Proc Natl Acad Sci U S A. 1973 Feb;70(2):361-5. doi: 10.1073/pnas.70.2.361.
4
Identification of a disulfide-linked procollagen as the biosynthetic precursor of chick-bone collagen.鉴定二硫键连接的前胶原作为鸡骨胶原的生物合成前体。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3521-5. doi: 10.1073/pnas.70.12.3521.
5
Ascorbate-dependent differences in the hydroxylation of proline and lysine in collagen synthesized by 3T6 fibroblasts in culture.培养的3T6成纤维细胞合成的胶原蛋白中脯氨酸和赖氨酸羟基化过程中依赖抗坏血酸的差异。
Biochim Biophys Acta. 1972 Oct 31;278(3):610-6. doi: 10.1016/0005-2795(72)90025-6.
6
Identification of the collagenous proteins synthesized by cultured cells from human skin.人皮肤培养细胞合成的胶原蛋白的鉴定。
Biochemistry. 1975 Apr 22;14(8):1589-94. doi: 10.1021/bi00679a007.
7
Biochemical characterization of collagens and of a non-collagenous protein synthesized by guinea pig lung fibroblasts in culture.豚鼠肺成纤维细胞在培养中合成的胶原蛋白和一种非胶原蛋白的生化特性
Connect Tissue Res. 1983;12(1):17-31. doi: 10.3109/03008208309005608.
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Collagen and procollagen production by a clonal line of Schwann cells.雪旺细胞克隆系产生胶原蛋白和前胶原蛋白
Proc Natl Acad Sci U S A. 1973 Jul;70(7):1943-6. doi: 10.1073/pnas.70.7.1943.
9
The synthesis and secretion of cartilage procollagen.软骨前胶原的合成与分泌。
Biochem J. 1975 Apr;148(1):129-38. doi: 10.1042/bj1480129.
10
Cell-free synthesis of procollagen: L-929 fibroblasts as a cellular model for dermatosparaxis.原胶原蛋白的无细胞合成：以L-929成纤维细胞作为皮肤松弛症的细胞模型
Proc Natl Acad Sci U S A. 1973 May;70(5):1378-82. doi: 10.1073/pnas.70.5.1378.

引用本文的文献

1
Biochemical and immunological studies of fibroblasts derived from a patient with Ehlers-Danlos syndrome type IV. Demonstrate reduced type III collagen synthesis.对一名患有IV型埃勒斯-当洛综合征患者的成纤维细胞进行的生化和免疫学研究。结果表明III型胶原蛋白合成减少。
Arch Dermatol Res. 1980;269(2):169-77. doi: 10.1007/BF00406537.
2
Case report and study of collagen metabolism in Marfan's syndrome.马凡综合征中胶原代谢的病例报告及研究
Klin Wochenschr. 1981 Jan 15;59(2):83-90. doi: 10.1007/BF01477287.
3
Type-specific collagen degradation by eosinophils.嗜酸性粒细胞对特定类型胶原蛋白的降解
Biochem J. 1982 Dec 1;207(3):621-4. doi: 10.1042/bj2070621.
4
Influence of cell density on collagen biosynthesis in fibroblast cultures.细胞密度对成纤维细胞培养中胶原蛋白生物合成的影响。
Biochem J. 1982 Sep 15;206(3):505-10. doi: 10.1042/bj2060505.
5
A defect in the structure of type I procollagen in a patient who had osteogenesis imperfecta: excess mannose in the COOH-terminal propeptide.一名患有成骨不全症患者的I型前胶原结构缺陷：羧基末端前肽中存在过量甘露糖。
Proc Natl Acad Sci U S A. 1980 Oct;77(10):6179-83. doi: 10.1073/pnas.77.10.6179.
6
Thermal stability of type I and type III procollagens from normal human fibroblasts and from a patient with osteogenesis imperfecta.来自正常人成纤维细胞和一名成骨不全症患者的I型和III型前胶原的热稳定性。
Proc Natl Acad Sci U S A. 1980 Jan;77(1):162-6. doi: 10.1073/pnas.77.1.162.
7
Type III collagen deficiency with normal phenotype.具有正常表型的III型胶原蛋白缺乏症。
J R Soc Med. 1983 Jun;76(6):518-20.
8
Comparison of collagen and glycosaminoglycan synthesis in attaching control and diabetic human skin fibroblasts.附着的对照和糖尿病患者皮肤成纤维细胞中胶原蛋白和糖胺聚糖合成的比较。
In Vitro. 1980 Feb;16(2):159-67. doi: 10.1007/BF02831506.
9
Amniocyte collagen synthesis does not distinguish Duchenne muscular dystrophy.羊膜细胞胶原蛋白合成无法区分杜氏肌营养不良症。
Am J Hum Genet. 1984 Nov;36(6):1397-8.
10
Lethal osteogenesis imperfecta congenita and a 300 base pair gene deletion for an alpha 1(I)-like collagen.致死性先天性成骨不全与α1（I）型胶原样基因的300个碱基对缺失
Br Med J (Clin Res Ed). 1984 Feb 11;288(6415):431-4. doi: 10.1136/bmj.288.6415.431.

本文引用的文献

1
Continuous scintillation counting of carbon-14 and tritium in effluent of the automatic amino acid analyzer.自动氨基酸分析仪流出物中碳-14和氚的连续闪烁计数
Anal Biochem. 1962 Dec;4:444-58. doi: 10.1016/0003-2697(62)90126-4.
2
Further evidence for a transport form of collagen. Its extrusion and extracellular conversion to tropocollagen in embryonic tendon.胶原蛋白转运形式的进一步证据。其在胚胎肌腱中的挤出及细胞外转化为原胶原蛋白。
FEBS Lett. 1971 Oct 1;17(2):245-248. doi: 10.1016/0014-5793(71)80156-4.
3
Inhibition of proline and lysine hydroxylation prevents normal extrusion of collagen by 3T6 fibroblasts in culture.脯氨酸和赖氨酸羟基化的抑制作用会阻止培养中的3T6成纤维细胞正常排出胶原蛋白。
FEBS Lett. 1971 Jun 2;15(1):59-64. doi: 10.1016/0014-5793(71)80079-0.
4
Molecular weight determination of random coil polypeptides from collagen by molecular sieve chromatography.通过分子筛色谱法测定胶原蛋白中无规卷曲多肽的分子量
Anal Biochem. 1968 Nov;26(2):305-12. doi: 10.1016/0003-2697(68)90342-4.
5
Biosynthesis of the alpha chains of collagen studied by pulse-labeling in culture.通过培养中的脉冲标记研究胶原蛋白α链的生物合成。
J Biol Chem. 1970 Nov 25;245(22):6201-7.
6
Collagen biosynthesis: synthesis and secretion of a high molecular weight collagen precursor (procollagen).胶原蛋白生物合成：高分子量胶原蛋白前体（前胶原）的合成与分泌。
Proc Natl Acad Sci U S A. 1971 Nov;68(11):2638-42. doi: 10.1073/pnas.68.11.2638.
7
Collagen synthesis on polysomes in vivo and in vitro.体内和体外多核糖体上的胶原蛋白合成
Nat New Biol. 1971 Jul 14;232(28):37-40. doi: 10.1038/newbio232037a0.
8
The nature of the collagen synthesized by cultured human fibroblasts.培养的人成纤维细胞合成的胶原蛋白的性质。
Proc Natl Acad Sci U S A. 1971 Feb;68(2):454-8. doi: 10.1073/pnas.68.2.454.
9
Collagen made of extended -chains, procollagen, in genetically-defective dermatosparaxic calves.基因缺陷的皮肤松垂症小牛中由伸展链组成的胶原蛋白，即前胶原。
Eur J Biochem. 1971 Dec 10;23(3):533-43. doi: 10.1111/j.1432-1033.1971.tb01651.x.
10
Studies on protocollagen: identification of a precursor of proto alpha 1.原胶原蛋白研究：原α1前体的鉴定
Biochem Biophys Res Commun. 1971 Jul 2;44(1):110-7. doi: 10.1016/s0006-291x(71)80165-1.

培养的人成纤维细胞中前胶原的产生。

Production of procollagen by human fibroblasts in culture.

作者信息

Smith B D, Byers P H, Martin G R

出版信息

Proc Natl Acad Sci U S A. 1972 Nov;69(11):3260-2. doi: 10.1073/pnas.69.11.3260.

DOI:10.1073/pnas.69.11.3260
PMID:4508318
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389749/
Abstract

Three hydroxyproline-containing proteins secreted into the medium by human fibroblasts in culture were isolated and characterized. A minor fraction was identical to the collagen monomer. The major fraction was a form of procollagen, which contained, in addition to pro alpha and alpha chains, a component estimated to have a molecular weight of 250,000. This component was a dimer of pro alpha chains joined by disulfide bonds. The third fraction, much lower in hydroxyproline and hydroxylysine content, was of still greater size. Pro alpha chains were released upon denaturation and reduction, indicating that this fraction may contain pro alpha chains linked by disulfide bonds to noncollagenous material.

摘要

对培养的人成纤维细胞分泌到培养基中的三种含羟脯氨酸的蛋白质进行了分离和鉴定。一小部分与胶原蛋白单体相同。主要部分是一种前胶原形式，除了原α链和α链外，还含有一种估计分子量为250,000的成分。该成分是由二硫键连接的原α链二聚体。第三部分的羟脯氨酸和羟赖氨酸含量低得多，分子量更大。变性和还原后会释放出原α链，表明该部分可能包含通过二硫键与非胶原物质相连的原α链。