Walther B T, Ohman R, Roseman S
Proc Natl Acad Sci U S A. 1973 May;70(5):1569-73. doi: 10.1073/pnas.70.5.1569.
Intercellular adhesion is measured by a new method based on determination of the rates of attachment of single cells to confluent cell monolayers. The procedure is simple, rapid, and reproducible. Specific and nonspecific intercellular adhesions can be quantitated and distinguished from each other, and from the adhesion to glass (or plastic). The rate of adhesion of single cells to the monolayer is characteristic of more than 80% of the single-cell population. This method, therefore, provides a means for study of the molecular basis of intercellular adhesion.
细胞间黏附通过一种基于测定单个细胞与汇合细胞单层附着速率的新方法来测量。该程序简单、快速且可重复。特异性和非特异性细胞间黏附可以进行定量并相互区分，也能与对玻璃（或塑料）的黏附区分开来。超过80%的单细胞群体具有单个细胞与单层的黏附速率特征。因此，该方法为研究细胞间黏附的分子基础提供了一种手段。
