Oppenheimer S B, Edidin M, Orr C W, Roseman S
Proc Natl Acad Sci U S A. 1969 Aug;63(4):1395-402. doi: 10.1073/pnas.63.4.1395.
Intercellular adhesion presumably involves components of the cell surface, but the chemical nature of these substances is not known. The present studies suggest that complex carbohydrates are required for the adhesion of at least one type of animal cell. Single cells obtained from "embryoid bodies," the ascites-grown form of a mouse teratoma, aggregated in a complex tissue culture medium, but not in a glucose balanced salts solution. The active component of the tissue culture medium was identified as L-glutamine, and the only compounds found to replace it were the hexosamines D-glucosamine and D-mannosamine. A variety of studies indicated that the three compounds were active as a consequence of metabolic reactions. These results are consistent with known metabolic pathways and indicate that the conversion of nonadhesive to adhesive teratoma cells requires the synthesis of glycoproteins, glycolipids, and/or polysaccharides.
细胞间黏附大概涉及细胞表面的成分，但这些物质的化学性质尚不清楚。目前的研究表明，复杂碳水化合物是至少一种动物细胞黏附所必需的。从小鼠畸胎瘤的腹水生长形式“胚状体”中获得的单细胞，在复杂的组织培养基中聚集，但在葡萄糖平衡盐溶液中不聚集。组织培养基的活性成分被鉴定为L-谷氨酰胺，唯一被发现可替代它的化合物是己糖胺D-葡萄糖胺和D-甘露糖胺。各种研究表明，这三种化合物由于代谢反应而具有活性。这些结果与已知的代谢途径一致，表明非黏附性畸胎瘤细胞向黏附性细胞的转化需要糖蛋白、糖脂和/或多糖的合成。
