用于增强疱疹病毒分离的组织外植体。
Tissue explants used for enhancement of herpesvirus isolations.
作者信息
Kalter S S, Eichberg J, Heberling R L, Felsburg P J
出版信息
Appl Microbiol. 1973 Feb;25(2):266-9. doi: 10.1128/am.25.2.266-269.1973.
Abstract
Herpesvirus recovery from normal and experimentally infected simian tissues prepared into 10 to 20% homogenates was compared with that obtained by explanting these same tissues. The explant procedure was by far the more sensitive of the two methods. As a result, 8 herpesvirus isolations were obtained by conventional virus isolation procedures in contrast to 74 isolates when explants were made from these same tissue sources. There is also evidence to suggest that the explant method is more sensitive than the use of tissue homogenates for recovery of other viruses as well.
摘要
将从正常和实验感染的猿猴组织制备成10%至20%匀浆后所回收的疱疹病毒，与通过将这些相同组织进行外植培养所获得的疱疹病毒进行了比较。在这两种方法中，外植培养法的敏感性要高得多。结果，通过传统病毒分离程序获得了8株疱疹病毒分离株，而从这些相同组织来源进行外植培养时则获得了74株分离株。还有证据表明，外植培养法在回收其他病毒方面也比使用组织匀浆更为敏感。
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