Rajcáni J, Ciampor F, Sabó A, Líbiková H, Rosenbergová M
Arch Virol. 1977;53(1-2):55-69. doi: 10.1007/BF01314847.
More than fifty albino rabbits were inoculated into the right scarified cornea with 10(7) PFU of the Kupka strain of human herpes virus type 1 (HHV-1). At intervals ranging from 4--280 days post infection (p.i.), both gasserian ganglia, both trigeminal nerve trunks and pieces from brain stem and from both corneas were explanted. Activation of the latent HHV-1 was found mainly in the homolateral ganglion tissue, but also in explants originating from the opposite ganglia. Within 24--72 hours, prior to the release of virus into the medium, one infectious unit of HHV was recovered from 10(4)--10(5) cells of the ganglion explant. In addition, a few neurons and satellite cells revealed the presence of virus-specific antigens when the explants were examined by immunofluorescence in serial sections. If the gangia were explanted in the presence of immune serum, the virus recovery rate was at least twice lower as compared to the virus activation in explants kept in the absence of immune serum.
用10⁷空斑形成单位（PFU）的1型人类疱疹病毒（HHV-1）库普卡毒株接种五十多只白化兔的右侧划痕角膜。在感染后4至280天的不同时间间隔，取出双侧三叉神经节、双侧三叉神经干以及脑干和双侧角膜的组织块。潜伏的HHV-1的激活主要在同侧神经节组织中发现，但也在来自对侧神经节的外植体中发现。在病毒释放到培养基之前的24至72小时内，从神经节外植体的10⁴至10⁵个细胞中可回收一个HHV感染单位。此外，当通过连续切片免疫荧光检查外植体时，一些神经元和卫星细胞显示存在病毒特异性抗原。如果在免疫血清存在的情况下取出神经节，与在无免疫血清情况下保存的外植体中的病毒激活相比，病毒回收率至少低两倍。
