中性肝果糖1,6 - 二磷酸酶向碱性的转变：酶分子特性的变化
Conversion of neutral to alkaline liver fructose 1,6-bisphosphatase: changes in molecular properties of the enzyme.
作者信息
Pontremoli S, Melloni E, De Flora A, Horecker B L
出版信息
Proc Natl Acad Sci U S A. 1973 Mar;70(3):661-4. doi: 10.1073/pnas.70.3.661.
Abstract
Removal of the NH(2)-terminal region of fructose 1,6-bisphosphatase from rabbit liver by digestion with subtillisin, or changes in conformation in this region of the protein produced by exposure to low concentrations of urea, result in similar changes in catalytic and allosteric properties of the enzyme. These changes include shift of the pH optimum to more alkaline pH, and loss of sensitivity to inhibition by AMP. The conformation changes are monitored by changes in the fluorescence of the single tryptophan residue, which is located near the NH(2)-terminus. Thus, the tryptophan-containing peptide appears to determine the functional properties of the native enzyme.
摘要
用枯草杆菌蛋白酶消化去除兔肝果糖1,6 -二磷酸酶的NH(2)-末端区域，或通过暴露于低浓度尿素使该蛋白质区域的构象发生变化，都会导致该酶的催化和别构性质发生类似变化。这些变化包括最适pH向更碱性的pH偏移，以及对AMP抑制的敏感性丧失。构象变化通过位于NH(2)-末端附近的单个色氨酸残基荧光的变化来监测。因此，含色氨酸的肽段似乎决定了天然酶的功能特性。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
ALTERATION OF ENZYMATIC ACTIVITIES OF RAT LIVER DIPHOSPHATASE BY TREATMENT WITH PAPAIN OR UREA.
Biochem Biophys Res Commun. 1963 Aug 1;12:305-9. doi: 10.1016/0006-291x(63)90301-2.
3
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定分子量的可靠性。
J Biol Chem. 1969 Aug 25;244(16):4406-12.
4
The tryptophan microenvironments in apomyoglobin.脱辅基肌红蛋白中的色氨酸微环境。
J Biol Chem. 1970 Dec 10;245(23):6300-6.
5
The carboxyl-terminal structure of rabbit liver aldolase (aldolase B).
J Biol Chem. 1970 Apr 25;245(8):2140-1.
6
Rabbit liver fructose 1,6-diphosphatase. Properties of the native enzyme and their modification by subtilisin.兔肝果糖1,6 - 二磷酸酶。天然酶的性质及其被枯草杆菌蛋白酶的修饰作用
Arch Biochem Biophys. 1972 Mar;149(1):222-31. doi: 10.1016/0003-9861(72)90317-7.
7
Fructose 1, 6-diphosphatase from liver: isolation of the native form with optimal activity at neutral pH.来自肝脏的果糖1,6-二磷酸酶：在中性pH下具有最佳活性的天然形式的分离。
Arch Biochem Biophys. 1971 Sep;146(1):161-6. doi: 10.1016/s0003-9861(71)80052-8.
8
Chemical modification of the allosteric and catalytic sites of fructose 1,6-diphosphatase.果糖1,6 - 二磷酸酶变构位点和催化位点的化学修饰。
Proc Natl Acad Sci U S A. 1966 May;55(5):1156-61. doi: 10.1073/pnas.55.5.1156.
9
Fructose diphosphatase from rabbit liver. 3. Nature of the groups reactive with dinitrofluorobenzene.
J Biol Chem. 1965 Sep;240(9):3469-72.
10
Fructose diphosphatase from rabbit liver. II. Changes in catalytic properties induced by dinitrofluorobenzene.
J Biol Chem. 1965 Sep;240(9):3464-8.
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