Rosenthal L J, Zamecnik P C
Proc Natl Acad Sci U S A. 1973 Mar;70(3):865-9. doi: 10.1073/pnas.70.3.865.
Using a chemical isotope derivative method, we have determined the minor base composition of the "70S-associated" 4S RNA isolated from avian myeloblastosis virus. The minor base composition of this "70S-associated" 4S RNA is strikingly similar to that of the corresponding "free" 4S RNA of the virion. This minor base content, plus the capacity to esterify amino acids, establishes that both of these virion 4S RNA fractions contain transfer RNA. The percentage of minor bases in virion "70S-associated" 4S RNA is, however, much lower than in "free" 4S virion RNA and in myeloblast 4S RNA. The implication is that the "70S-associated" 4S RNA fraction as isolated herein also contains RNA that is not transfer RNA. This latter RNA may represent either degradation products of high molecular weight RNA or indigenous 4S RNA of undetermined function.
我们使用化学同位素衍生法,测定了从禽成髓细胞瘤病毒中分离出的“与70S相关的”4S RNA的稀有碱基组成。这种“与70S相关的”4S RNA的稀有碱基组成与病毒体相应的“游离”4S RNA的稀有碱基组成惊人地相似。这种稀有碱基含量,加上酯化氨基酸的能力,表明这两种病毒体4S RNA组分都含有转运RNA。然而,病毒体“与70S相关的”4S RNA中稀有碱基的百分比远低于“游离”病毒体4S RNA和成髓细胞4S RNA中的百分比。这意味着本文分离出的“与70S相关的”4S RNA组分还含有不是转运RNA的RNA。后一种RNA可能代表高分子量RNA的降解产物或功能未明的天然4S RNA。