通过碱性氯化铯密度沉降从鸡DNA中分离与禽成髓细胞瘤病毒RNA互补的DNA序列。
Separation of DNA sequences complementary to the RNA of avian myeloblastosis virus from chicken DNA by alkaline cesium chloride density sedimentation.
作者信息
Shoyab M, Baluda M A
出版信息
J Virol. 1973 Sep;12(3):534-7. doi: 10.1128/JVI.12.3.534-537.1973.
Abstract
Density gradient sedimentation in alkaline cesium chloride of DNA from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13S revealed that the DNA sequences complementary to 70S avian myeloblastosis virus RNA sedimented in the high guanine plus cytosine region ahead of the main peak of cellular DNA. When the DNA was fragmented into pieces of 6.6S there was a broader distribution of the DNA sequences complementary to the viral RNA. This technique could be employed as a step towards the isolation of DNA copies of the entire viral RNA genome from the mass of host cellular DNA.
摘要
将来自正常鸡胚胎或白血病成髓细胞的DNA片段化至13S大小后，在碱性氯化铯中进行密度梯度沉降分析，结果显示，与70S禽成髓细胞瘤病毒RNA互补的DNA序列沉降在细胞DNA主峰之前的高鸟嘌呤加胞嘧啶区域。当DNA片段化至6.6S时，与病毒RNA互补的DNA序列分布更为广泛。该技术可作为从大量宿主细胞DNA中分离整个病毒RNA基因组DNA拷贝的一个步骤。
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本文引用的文献
1
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.DNA大小与形状的沉降研究
J Mol Biol. 1965 Feb;11:373-90. doi: 10.1016/s0022-2836(65)80064-x.
2
Forms of deoxyribonucleic acid produced by virions of the ribonucleic acid tumor viruses.由核糖核酸肿瘤病毒的病毒粒子产生的脱氧核糖核酸形式。
J Virol. 1971 Jan;7(1):106-11. doi: 10.1128/JVI.7.1.106-111.1971.
3
Composition and structure of chromosomal and amplified ribosomal DNA's of Xenopus laevis.非洲爪蟾染色体和扩增核糖体DNA的组成与结构
J Mol Biol. 1970 Jul 28;51(2):341-60. doi: 10.1016/0022-2836(70)90147-6.
4
Specific gene amplification in oocytes. Oocyte nuclei contain extrachromosomal replicas of the genes for ribosomal RNA.卵母细胞中的特定基因扩增。卵母细胞核含有核糖体RNA基因的染色体外复制物。
Science. 1968 Apr 19;160(3825):272-80. doi: 10.1126/science.160.3825.272.
5
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J Virol. 1972 Nov;10(5):1002-9. doi: 10.1128/JVI.10.5.1002-1009.1972.
6
Widespread presence, in chickens, of DNA complementary to the RNA genome of avian leukosis viruses.鸡体内广泛存在与禽白血病病毒RNA基因组互补的DNA。
Proc Natl Acad Sci U S A. 1972 Mar;69(3):576-80. doi: 10.1073/pnas.69.3.576.
7
Extent of transcription by the RNA-dependent DNA polymerase of Rous sarcoma virus.劳氏肉瘤病毒的RNA依赖性DNA聚合酶的转录范围。
Nat New Biol. 1971 Sep 1;233(35):19-21. doi: 10.1038/newbio233019a0.
8
Nucleotide composition of RNA hybridized to homologous DNA from cells transformed by avian tumour viruses.与禽肿瘤病毒转化细胞的同源DNA杂交的RNA的核苷酸组成
Nat New Biol. 1971 May 19;231(20):90-1. doi: 10.1038/newbio231090a0.
9
Heterogeneity of murine leukemia virus in vitro DNA; detection of viral DNA in mammalian cells.
Science. 1971 Jun 25;172(3990):1353-5. doi: 10.1126/science.172.3990.1353.
10
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J Virol. 1971 Jan;7(1):77-86. doi: 10.1128/JVI.7.1.77-86.1971.
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