Gallagher R E, Gallo R C
J Virol. 1973 Sep;12(3):449-57. doi: 10.1128/JVI.12.3.449-457.1973.
Avian myeloblastosis virus (AMV) 4S RNA was tested for amino acid acceptor activity for 18 of the 20 amino acids. A nonrandom distribution of viral tRNAs was found compared with tRNA from normal liver or from AMV-infected leukemic myeloblasts, confirming previous reports. Methionine and proline tRNAs were considerably enriched, whereas glutamic acid, glutamine, serine, tyrosine, and valine tRNAs were markedly depleted in AMV relative to homologous cellular tRNAs. The seven AMV tRNAs with the greatest amino acid acceptance capacities, which were in order methionine, proline, lysine, arginine, histidine, isoleucine, and threonine tRNAs, were compared with homologous tRNAs from leukemic myeloblasts and liver by reversed-phase 5 chromatography. Of the 25 isoaccepting chromatographic fractions identified, no tRNA species unique to AMV was detected. Only methionyl-tRNA showed a substantial quantitative variation in isoaccepting species compared with the host cell. Thus, viral selectivity for amino acid-specific tRNAs is not, generally, paralleled by selectivity for individual isoaccepting tRNA species. Qualitative differences in arginyl- and histidyl-tRNA isoaccepting species were discovered in virus and leukemic myeloblasts compared with liver. This indicates the existence of structural differences in these tRNA species which could be related to virus replication or expression.
对禽成髓细胞瘤病毒（AMV）4S RNA进行了检测，以确定其对20种氨基酸中18种氨基酸的氨酰基接受活性。与正常肝脏或AMV感染的白血病成髓细胞中的tRNA相比，发现病毒tRNA的分布是非随机的，这证实了先前的报道。相对于同源细胞tRNA，甲硫氨酸和脯氨酸tRNA显著富集，而谷氨酸、谷氨酰胺、丝氨酸、酪氨酸和缬氨酸tRNA在AMV中明显减少。通过反相5色谱法，将具有最大氨酰基接受能力的七种AMV tRNA，即按顺序为甲硫氨酸、脯氨酸、赖氨酸、精氨酸、组氨酸、异亮氨酸和苏氨酸tRNA，与白血病成髓细胞和肝脏中的同源tRNA进行了比较。在鉴定出的25个同功受体色谱组分中，未检测到AMV特有的tRNA种类。与宿主细胞相比，只有甲硫氨酰-tRNA在同功受体种类上表现出显著的定量差异。因此，病毒对氨基酸特异性tRNA的选择性通常与对单个同功受体tRNA种类的选择性并不平行。与肝脏相比，在病毒和白血病成髓细胞中发现了精氨酰-tRNA和组氨酰-tRNA同功受体种类的定性差异。这表明这些tRNA种类存在结构差异，可能与病毒复制或表达有关。
