模板薄膜凝胶扩散法中抗血清的孔内吸收
Intrawell absorption of antisera in a template thin-film gel diffusion method.
作者信息
Auernheimer A H, Zimmerman R A
出版信息
Appl Microbiol. 1973 Jun;25(6):917-20. doi: 10.1128/am.25.6.917-920.1973.
Abstract
This report describes a method for in situ absorption of antisera within the template wells used in thin-film microgel diffusion. The intrawell absorption of antisera offers several advantages over previously reported techniques for detecting the presence of given antigens in biological preparations. The procedure is time saving, antigens are conserved, optimum antigen-antibody ratios are easily determined, and tests of several different absorbing antigens can be made on a single plate.
摘要
本报告描述了一种在薄膜微凝胶扩散所用模板孔内原位吸收抗血清的方法。与先前报道的检测生物制剂中特定抗原存在的技术相比，抗血清的孔内吸收具有几个优点。该程序节省时间，抗原得以保存，易于确定最佳抗原-抗体比例，并且可以在单个平板上对几种不同的吸收性抗原进行检测。
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本文引用的文献
1
Specific inhibition of precipitation as an aid in antigen analysis with gel diffusion method.
Proc Soc Exp Biol Med. 1952 Feb;79(2):319-24. doi: 10.3181/00379727-79-19364.
2
Precipitate patterns in agar gel with sera from human amebiasis and Entamoeba histolytica antigen.
J Parasitol. 1963 Apr;49:313-5.
3
A slide microtechnique for the analysis of immune precipitates in gel.
Int Arch Allergy Appl Immunol. 1957;10(6):355-60. doi: 10.1159/000228394.
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