Harrell W K, George J R
Appl Microbiol. 1972 Jun;23(6):1047-52. doi: 10.1128/am.23.6.1047-1052.1972.
A comparative study was made of the single radial immunodiffusion test and the classical quantitative precipitin test for determining the amount of precipitable antibodies present in streptococcal groups A and C antisera. The potency of 21 group A and 54 group C antisera was determined by both methods; purified group-specific carbohydrates were used as antigens. The coefficient of correlation between the results from the two methods was 0.976 for group A antisera and 0.946 for group C antisera. When the concentration of antigen, the volume of antiserum used, and the depth of the antigen-agar mixture are kept constant, the diameter of the precipitin disc is directly related to the concentration of precipitable antibodies present in the antiserum. The use of the radial immunodiffusion test for evaluating and standardizing streptococcal grouping antisera is discussed as well as the advantages and disadvantages of using a concentrated vaccine for producing these antisera.
对单向放射免疫扩散试验和经典定量沉淀试验进行了比较研究，以确定A组和C组链球菌抗血清中可沉淀抗体的含量。用这两种方法测定了21份A组抗血清和54份C组抗血清的效价；使用纯化的组特异性碳水化合物作为抗原。两种方法所得结果之间的相关系数，A组抗血清为0.976，C组抗血清为0.946。当抗原浓度、所用抗血清体积以及抗原-琼脂混合物的深度保持恒定时，沉淀盘的直径与抗血清中可沉淀抗体的浓度直接相关。文中讨论了使用放射免疫扩散试验评估和标准化链球菌分组抗血清，以及使用浓缩疫苗生产这些抗血清的优缺点。
