人胎盘N-乙酰己糖胺酶A和B的碳水化合物组成。
Carbohydrate composition of human placental N-acetylhexosaminidase A and B.
作者信息
Freeze H, Geiger B, Miller A L
出版信息
Biochem J. 1979 Feb 1;177(2):749-52. doi: 10.1042/bj1770749.
Abstract
The carbohydrate composition of N-acetyl-beta-D-hexosaminidases (EC 3.2.1.52) A, B and heat-converted B was determined by g.l.c. Similar quantities of mannose, N-acetyl-glucosamine and galactose are present in the A and B isoenzymes, whereas N-acetyl-neuraminic acid is found in significant amount in only the A isoenzyme. The heat-converted hexosaminidase B also contains only trace amounts of N-acetylneuraminic acid, but is about 1.5-fold richer in mannose and N-acetylglucosamine and nearly 2-fold richer in galactose than native hexosaminidase B. Since native and converted hexosaminidase B are thought to be composed of four identical protein chains, our results suggest that there may be variable glycosylation of these chains.
摘要
通过气相色谱法测定了N-乙酰-β-D-己糖胺酶（EC 3.2.1.52）A、B以及热转化型B的碳水化合物组成。A和B同工酶中存在相似量的甘露糖、N-乙酰葡糖胺和半乳糖，而仅在A同工酶中发现大量的N-乙酰神经氨酸。热转化型己糖胺酶B也仅含有痕量的N-乙酰神经氨酸，但与天然己糖胺酶B相比，其甘露糖和N-乙酰葡糖胺含量约高1.5倍，半乳糖含量几乎高2倍。由于天然型和转化型己糖胺酶B被认为由四条相同的蛋白质链组成，我们的结果表明这些链可能存在可变糖基化。
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