一种蛋白酶抑制剂对体外恶性细胞侵袭的抑制作用。
Inhibition of malignant cell invasion in vitro by a proteinase inhibitor.
作者信息
Latner A L, Longstaff E, Pradhan K
出版信息
Br J Cancer. 1973 Jun;27(6):460-4. doi: 10.1038/bjc.1973.58.
Abstract
The inhibitory effect of the protease inhibitor aprotinin (Trasylol) on the invasion of mouse kidney explants by polyoma virus transformed BHK21 cells was investigated using a mixed cell/organ culture technique. The extent of invasion was monitored by following the changes in LDH isoenzyme pattern in the explants and by histological assessment. The kidney explants containing aprotinin were found to maintain a normal kidney LDH pattern and to suffer considerably less invasion than the explants not containing the drug. These results support the idea that proteolytic enzymes are associated with invasion and that inhibitors of protease activity could possibly be useful in the management of clinical cancer.
摘要
使用细胞/器官混合培养技术，研究了蛋白酶抑制剂抑肽酶（Trasylol）对多瘤病毒转化的BHK21细胞侵袭小鼠肾外植体的抑制作用。通过跟踪外植体中乳酸脱氢酶同工酶模式的变化和组织学评估来监测侵袭程度。发现含有抑肽酶的肾外植体维持正常的肾乳酸脱氢酶模式，并且比不含该药物的外植体遭受的侵袭要少得多。这些结果支持了蛋白水解酶与侵袭相关的观点，并且蛋白酶活性抑制剂可能在临床癌症管理中有用。
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