谷氨酸脱氢酶中组氨酸残基与焦碳酸二乙酯的反应。
The reaction of a histidine residue in glutamate dehydrogenase with diethyl pyrocarbonate.
作者信息
Wallis R B, Holbrook J J
出版信息
Biochem J. 1973 May;133(1):183-7. doi: 10.1042/bj1330183.
Abstract
- One mol of diethyl pyrocarbonate will react with one mol of glutamate dehydrogenase polypeptide chains to form one mol of N(1)-carbethoxyhistidine. Reaction is prevented by NADH. 2. The 1:1 complex has an increased specific activity (1.4-2.0-fold). 3. The reason for the activation is discussed. The results are not consistent with NADH dissociation from the enzyme-glutamate-NADH complex being rate-limiting in the steady state measured. 4. The effects of modification on the properties of the enzyme were investigated. The effects of GTP and NAD(+) on the enzyme activity are unaltered by activation. NADH binding is unaltered and there is no apparent change in the molecular weight. However, the activated enzyme can still be further activated by ADP. K(s) for ADP is decreased fivefold.
摘要
- 一摩尔焦碳酸二乙酯会与一摩尔谷氨酸脱氢酶多肽链反应，形成一摩尔N(1)-乙氧羰基组氨酸。NADH可阻止该反应。2. 1:1复合物的比活性增加（1.4至2.0倍）。3. 讨论了激活的原因。结果与在稳态测量中NADH从酶-谷氨酸-NADH复合物解离作为限速因素不一致。4. 研究了修饰对酶性质的影响。GTP和NAD(+)对酶活性的影响不受激活的改变。NADH结合未改变，分子量也无明显变化。然而，活化酶仍可被ADP进一步激活。ADP的K(s)降低了五倍。
相似文献
1
The reaction of a histidine residue in glutamate dehydrogenase with diethyl pyrocarbonate.谷氨酸脱氢酶中组氨酸残基与焦碳酸二乙酯的反应。
Biochem J. 1973 May;133(1):183-7. doi: 10.1042/bj1330183.
2
Effects of adenosine 5'-diphosphate on bovine glutamate dehydrogenase: diethyl pyrocarbonate modification.5'-二磷酸腺苷对牛谷氨酸脱氢酶的影响：焦碳酸二乙酯修饰
Biochemistry. 1980 Dec 23;19(26):6057-61. doi: 10.1021/bi00567a017.
3
Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase.猪心乳酸脱氢酶中一个必需组氨酸残基的离子特性
Biochem J. 1973 Apr;131(4):729-38. doi: 10.1042/bj1310729.
4
Adenosine 5'-0-[S-(4-succinimidyl-benzophenone)thiophosphate]: a new photoaffinity label of the allosteric ADP site of bovine liver glutamate dehydrogenase.腺苷 5'-O-[S-(4-琥珀酰亚胺基-二苯甲酮)硫代磷酸酯]：牛肝谷氨酸脱氢酶变构 ADP 位点的新型光亲和标记物。
Biochemistry. 2001 Feb 13;40(6):1577-86. doi: 10.1021/bi002336r.
5
The chemical reactivity of the histidine-195 residue in lactate dehydrogenase thiomethylated at the cysteine-165 residue.在半胱氨酸-165残基处硫甲基化的乳酸脱氢酶中组氨酸-195残基的化学反应性。
Biochem J. 1981 Jan 1;193(1):93-7. doi: 10.1042/bj1930093.
6
The effect of modifying lysine-126 on the physical, catalytic and regulatory properties of bovine liver glutamate dehydrogenase.修饰赖氨酸-126对牛肝谷氨酸脱氢酶物理、催化及调节特性的影响
Biochem J. 1973 May;133(1):173-82. doi: 10.1042/bj1330173.
7
Activation of bovine liver glutamate dehydrogenase by covalent reaction of adenosine 5'-O-[S-(4-bromo-2,3-dioxobutyl)thiophosphate] with arginine-459 at an ADP regulatory site.通过腺苷5'-O-[S-(4-溴-2,3-二氧代丁基)硫代磷酸酯]与ADP调节位点的精氨酸-459发生共价反应来激活牛肝谷氨酸脱氢酶。
Biochemistry. 1994 Sep 27;33(38):11544-53. doi: 10.1021/bi00204a017.
8
Chemical modification of 3 alpha,20 beta-hydroxysteroid dehydrogenase with diethyl pyrocarbonate. Evidence for an essential, highly reactive, lysyl residue.用焦碳酸二乙酯对3α,20β-羟基类固醇脱氢酶进行化学修饰。关于一个必需的、高反应性的赖氨酰残基的证据。
Biochemistry. 1987 Mar 10;26(5):1247-51. doi: 10.1021/bi00379a007.
9
10
Formation of transient complexes in the glutamate dehydrogenase catalyzed reaction.谷氨酸脱氢酶催化反应中瞬时复合物的形成。
Biochemistry. 1975 Nov 18;14(23):5094-8. doi: 10.1021/bi00694a011.
引用本文的文献
1
Determining composition of micron-scale protein deposits in neurodegenerative disease by spatially targeted optical microproteomics.通过空间靶向光学微蛋白质组学确定神经退行性疾病中微米级蛋白质沉积物的组成。
Elife. 2015 Sep 29;4:e09579. doi: 10.7554/eLife.09579.
2
Histidine residues and the enzyme activity of pig heart supernatant malate dehydrogenase.组氨酸残基与猪心上清液苹果酸脱氢酶的酶活性
Biochem J. 1974 Jun;139(3):797-800. doi: 10.1042/bj1390797.
3
Electrophoresis of human l-glutamate dehydrogenase: tissue distribution and preliminary population survey.人L-谷氨酸脱氢酶的电泳：组织分布及初步群体调查
Biochem Genet. 1977 Feb;15(1-2):87-91. doi: 10.1007/BF00484550.
本文引用的文献
1
The dependence of the substrate specificity on the conformation of crystalline glutamate dehydrogenase.
J Biol Chem. 1965 Oct;240(10):3793-8.
2
Evidence of a critical histidine residue in 6-phosphogluconate dehydrogenase from Candida utilis.产朊假丝酵母6-磷酸葡萄糖酸脱氢酶中关键组氨酸残基的证据。
Biochemistry. 1968 Apr;7(4):1514-8. doi: 10.1021/bi00844a038.
3
Essential histidyl residues of octopine dehydrogenase.章鱼碱脱氢酶的必需组氨酸残基。
Eur J Biochem. 1971 Jul 29;21(2):161-9. doi: 10.1111/j.1432-1033.1971.tb01452.x.
4
Identification of an essential, reactive histidine in pig heart mitochondrial malate dehydrogenase.
Eur J Biochem. 1970 Sep;15(3):562-7. doi: 10.1111/j.1432-1033.1970.tb01041.x.
5
Ethoxyformylation of proteins. Reaction of ethoxyformic anhydride with alpha-chymotrypsin, pepsin, and pancreatic ribonuclease at pH 4.蛋白质的乙氧基甲酰化。在pH值为4的条件下，乙氧基甲酸酐与α-胰凝乳蛋白酶、胃蛋白酶和胰腺核糖核酸酶的反应。
Biochemistry. 1970 Jan 20;9(2):251-8. doi: 10.1021/bi00804a010.
6
The site at which 4-iodoacetamidosalicylate reacts with glutamate dehydrogenases.4-碘乙酰氨基水杨酸与谷氨酸脱氢酶反应的位点。
Biochem J. 1973 May;133(1):165-71. doi: 10.1042/bj1330165.
7
[Transient complexes in the catalysis of glutamate dehydrogenase].[谷氨酸脱氢酶催化过程中的瞬时复合物]
Biochimie. 1971;53(2):153-9. doi: 10.1016/s0300-9084(71)80047-0.
8
Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase.猪心乳酸脱氢酶中一个必需组氨酸残基的离子特性
Biochem J. 1973 Apr;131(4):729-38. doi: 10.1042/bj1310729.
9
The effect of modifying lysine-126 on the physical, catalytic and regulatory properties of bovine liver glutamate dehydrogenase.修饰赖氨酸-126对牛肝谷氨酸脱氢酶物理、催化及调节特性的影响
Biochem J. 1973 May;133(1):173-82. doi: 10.1042/bj1330173.
10
Covalent attachment of diethylstilbestrol to glutamate dehydrogenase: implications for allosteric regulation.己烯雌酚与谷氨酸脱氢酶的共价结合：对别构调节的影响
Proc Natl Acad Sci U S A. 1971 May;68(5):916-9. doi: 10.1073/pnas.68.5.916.
Zotero 插件