Flory W, Koeppe R E
Biochem J. 1973 Jun;133(2):391-4. doi: 10.1042/bj1330391.
Rat liver pyruvate kinase, prepared by Sephadex treatment of a 10(5)g supernatant in phosphate buffer, is quite stable and gives reproducible results when a variety of parameters are altered in the enzyme assay. Incubation of this preparation at 25 degrees C or 0-2 degrees C has no effect on the activation by fructose 1,6-diphosphate or inhibition by ATP or alanine.
用葡聚糖凝胶处理磷酸盐缓冲液中的10(5)g大鼠肝脏上清液制备的丙酮酸激酶相当稳定,当酶测定中的各种参数改变时,能给出可重复的结果。将该制剂在25℃或0-2℃下孵育,对果糖1,6-二磷酸的激活或ATP或丙氨酸的抑制没有影响。