大鼠肝脏丙酮酸激酶缺乏温度敏感性。
Lack of temperature-sensitivity of rat liver pyruvate kinase.
作者信息
Flory W, Koeppe R E
出版信息
Biochem J. 1973 Jun;133(2):391-4. doi: 10.1042/bj1330391.
Abstract
Rat liver pyruvate kinase, prepared by Sephadex treatment of a 10(5)g supernatant in phosphate buffer, is quite stable and gives reproducible results when a variety of parameters are altered in the enzyme assay. Incubation of this preparation at 25 degrees C or 0-2 degrees C has no effect on the activation by fructose 1,6-diphosphate or inhibition by ATP or alanine.
摘要
用葡聚糖凝胶处理磷酸盐缓冲液中的10(5)g大鼠肝脏上清液制备的丙酮酸激酶相当稳定，当酶测定中的各种参数改变时，能给出可重复的结果。将该制剂在25℃或0-2℃下孵育，对果糖1,6-二磷酸的激活或ATP或丙氨酸的抑制没有影响。
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Biochem Biophys Res Commun. 1965 Oct 8;21(1):55-60. doi: 10.1016/0006-291x(65)90425-0.
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Some kinetic properties of liver pyruvate kinase (type L). II. Effect of pH on its allosteric behavior.肝脏丙酮酸激酶（L型）的一些动力学特性。II. pH对其别构行为的影响。
J Biol Chem. 1969 Jun 25;244(12):3142-7.
5
Some kinetic properties of liver pyruvate kinase (type L).肝脏丙酮酸激酶（L型）的一些动力学特性。
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6
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Biochem Biophys Res Commun. 1968 Jan 11;30(1):14-20. doi: 10.1016/0006-291x(68)90705-5.
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