Sato H, Murata R
Infect Immun. 1973 Sep;8(3):360-9. doi: 10.1128/iai.8.3.360-369.1973.
Clostridium perfringens was found to produce alpha toxin in a synthetic medium containing zinc; in medium containing no zinc, a little toxin was detected in the early logarithmic phase of growth and it disappeared rapidly. No intracellular accumulation of alpha toxin protein occurred whether or not zinc was present in the medium. In zinc-deficient medium, the organisms produced and released into the surrounding medium the protein specifically precipitable with alpha antitoxin in an amount comparable to that of alpha toxin produced in the zinc-containing medium. The protein combined rapidly in some unknown way with zinc to form the active and stable alpha toxin.
研究发现，产气荚膜梭菌在含有锌的合成培养基中产生α毒素；在不含锌的培养基中，在生长的对数早期阶段检测到少量毒素，且毒素迅速消失。无论培养基中是否存在锌，α毒素蛋白均未在细胞内积累。在缺锌培养基中，该菌产生并释放到周围培养基中的可被α抗毒素特异性沉淀的蛋白质，其含量与在含锌培养基中产生的α毒素相当。该蛋白质以某种未知方式迅速与锌结合，形成活性稳定的α毒素。
