乳酸脱氢酶在固定化核苷酸上的亲和层析
Affinity chromatography of lactate dehydrogenase on immobilized nucleotides.
作者信息
Lowe C R, Dean P D
出版信息
Biochem J. 1973 Jul;133(3):515-20. doi: 10.1042/bj1330515.
Abstract
The interaction of two isoenzymes of lactate dehydrogenase from pig heart muscle (H(4)) and rabbit skeletal muscle (M(4)), with immobilized nucleotides was examined: the effects of pH and temperature on the binding of lactate dehydrogenase were studied with immobilized NAD(+) matrices. The influence of substrate, product and sulphite on the binding of heart muscle lactate dehydrogenase to immobilized NAD(+) was investigated. The interaction of both lactate dehydrogenase isoenzymes with immobilized pyridine and adenine nucleotides and their derivatives were measured. The effects of these parameters on the interaction of lactate dehydrogenase with immobilized nucleotides were correlated with the known kinetic and molecular properties of the enzymes in free solution.
摘要
研究了猪心肌（H(4)）和兔骨骼肌（M(4)）乳酸脱氢酶的两种同工酶与固定化核苷酸的相互作用：用固定化NAD(+)基质研究了pH和温度对乳酸脱氢酶结合的影响。研究了底物、产物和亚硫酸盐对心肌乳酸脱氢酶与固定化NAD(+)结合的影响。测定了两种乳酸脱氢酶同工酶与固定化吡啶和腺嘌呤核苷酸及其衍生物的相互作用。这些参数对乳酸脱氢酶与固定化核苷酸相互作用的影响与游离溶液中酶已知的动力学和分子特性相关。
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本文引用的文献
1
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Biochemistry. 1964 Dec;3:1913-9. doi: 10.1021/bi00900a022.
2
Lactic dehydrogenase. IV. The influence of pH on the kinetics of the reaction.乳酸脱氢酶。IV。pH对反应动力学的影响。
J Biol Chem. 1958 Apr;231(2):1065-83.
3
Lactic dehydrogenase. II. Variation of kinetic and equilibrium constants with temperature.乳酸脱氢酶。二。动力学和平衡常数随温度的变化。
J Biol Chem. 1956 Jul;221(1):191-209.
4
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FEBS Lett. 1972 Sep 15;25(2):234-238. doi: 10.1016/0014-5793(72)80492-7.
5
Affinity chromatography of enzymes on insolubilized cofactors.
FEBS Lett. 1971 May 20;14(5):313-316. doi: 10.1016/0014-5793(71)80288-0.
6
Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
Nature. 1967 Jun 24;214(5095):1302-4. doi: 10.1038/2141302a0.
7
Comparative study of methods for quantitation of lactate dehydrogenase isozymes.
Anal Biochem. 1970 Aug;36(2):443-53. doi: 10.1016/0003-2697(70)90381-7.
8
The effective use of affinity chromatogrphy for the study of complex formation of bovine carboxypeptidase B with basic and aromatic amino acid analogues.
Biochem Biophys Res Commun. 1971 Oct 1;45(1):27-33. doi: 10.1016/0006-291x(71)90045-3.
9
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10
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Biochemistry. 1972 Feb 1;11(3):442-8. doi: 10.1021/bi00753a023.
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