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基于固定化辅因子的烟酰胺核苷酸依赖性脱氢酶的纯化

The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.

作者信息

Lowe C R, Harvey M J, Craven D B, Kerfoot M A, Hollows M E, Dean P D

出版信息

Biochem J. 1973 Jul;133(3):507-13. doi: 10.1042/bj1330507.

DOI:10.1042/bj1330507
PMID:4147413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1177728/
Abstract
  1. The general applicability of affinity chromatography to the purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors is illustrated with several examples taken from crude systems. 2. Methods for overcoming the inevitable loss of selectivity experienced with these polymers are suggested. Effective use of the appropriate nucleotide, the second substrate and other interacting ligands can be made to selectively alter the chromatographic behaviour of the desired enzyme.
摘要
  1. 亲和色谱法在固定化辅因子上纯化烟酰胺核苷酸依赖性脱氢酶的一般适用性通过从粗体系中选取的几个例子来说明。2. 提出了克服这些聚合物不可避免的选择性损失的方法。可以有效地利用合适的核苷酸、第二底物和其他相互作用配体来选择性地改变所需酶的色谱行为。

相似文献

1
The purification of nicotinamide nucleotide-dependent dehydrogenases on immobilized cofactors.基于固定化辅因子的烟酰胺核苷酸依赖性脱氢酶的纯化
Biochem J. 1973 Jul;133(3):507-13. doi: 10.1042/bj1330507.
2
Some applications of insolubilised cofactors to the purification of pyridine nucleotide-dependent dehydrogenases.不溶性辅因子在吡啶核苷酸依赖性脱氢酶纯化中的一些应用。
Biochem Biophys Res Commun. 1972 Aug 21;48(4):1004-10. doi: 10.1016/0006-291x(72)90708-5.
3
Use of differently immobilized nucleotides for binding NAD+-dependent dehydrogenases.使用不同固定化核苷酸结合NAD+依赖性脱氢酶。
Methods Enzymol. 1980;66:192-208. doi: 10.1016/0076-6879(80)66459-3.
4
Affinity chromatography of nicotinamide nucleotide-dependent dehydrogenases on immobilized nucleotide derivatives.固定化核苷酸衍生物上烟酰胺核苷酸依赖性脱氢酶的亲和层析
Biochem J. 1974 Sep;141(3):775-87. doi: 10.1042/bj1410775.
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Rapid separation of dehydrogenases by affinity chromatography with new induced specificity phases.利用具有新诱导特异性相的亲和色谱法快速分离脱氢酶。
Biochimie. 1986 Apr;68(4):565-8. doi: 10.1016/s0300-9084(86)80200-0.
6
Purification and separation of pyridine nucleotide-linked dehydrogenases by affinity chromatography techniques.通过亲和色谱技术纯化和分离吡啶核苷酸连接的脱氢酶。
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3450-4. doi: 10.1073/pnas.71.9.3450.
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Study of the interaction between NADP-dependent dehydrogenase and immobilized adenosine 2'-monophosphate by means of affinity electrophoresis.
J Chromatogr. 1980 Aug 1;196(1):85-99. doi: 10.1016/s0021-9673(00)80361-1.
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Purification of nicotinamide-adenine dinucleotide-dependent dehydrogenases by affinity chromatography.通过亲和色谱法纯化烟酰胺腺嘌呤二核苷酸依赖性脱氢酶。
Biochem J. 1971 Nov;125(2):20P-21P. doi: 10.1042/bj1250020p.
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A "stripping" ligand tactic for use with the kinetic locking-on strategy: its use in the resolution and bioaffinity chromatographic purification of NAD(+)-dependent dehydrogenases.一种与动力学锁定策略配合使用的“剥离”配体策略：其在NAD(+)依赖性脱氢酶的拆分及生物亲和色谱纯化中的应用
Protein Expr Purif. 1999 Aug;16(3):424-31. doi: 10.1006/prep.1999.1089.
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Affinity chromatography of nicotinamide-adenine dinucleotide-linked dehydrogenases on immobilized derivatives of the dinucleotide.烟酰胺腺嘌呤二核苷酸连接的脱氢酶在二核苷酸固定化衍生物上的亲和层析
Biochem J. 1973 Dec;135(4):595-607. doi: 10.1042/bj1350595.

引用本文的文献

1
Affinity chromatography of nicotinamide nucleotide-dependent dehydrogenases on immobilized nucleotide derivatives.固定化核苷酸衍生物上烟酰胺核苷酸依赖性脱氢酶的亲和层析
Biochem J. 1974 Sep;141(3):775-87. doi: 10.1042/bj1410775.
2
Preparation of adenosine nucleotide derivatives suitable for affinity chromatography.适用于亲和色谱的腺苷核苷酸衍生物的制备。
Biochem J. 1974 Jun;139(3):609-23. doi: 10.1042/bj1390609.
3
The immobilization of adenine nucleotides on polysaccharides by using glutaraldehyde coupling and borohydride reduction.通过戊二醛偶联和硼氢化钠还原将腺嘌呤核苷酸固定在多糖上。
Biochem J. 1978 Oct 1;175(1):349-52. doi: 10.1042/bj1750349.
4
An examination of potential matrices for the affinity chromatography of NADP+-linked dehydrogenases with special reference to 6-phosphogluconate dehydrogenase.对用于NADP +连接的脱氢酶亲和色谱的潜在基质的研究，特别参考6-磷酸葡萄糖酸脱氢酶。
Biochem J. 1977 Mar 1;161(3):561-8. doi: 10.1042/bj1610561.

本文引用的文献

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A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS.一种用于蛋白质定量的微量双缩脲法。
Anal Biochem. 1964 Dec;9:401-10. doi: 10.1016/0003-2697(64)90200-3.
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Affinity chromatography of lactate dehydrogenase Model studies demonstrating the potential of the technique in the mechanistic investigation as well as in the purification of multi-substrate enzymes.乳酸脱氢酶的亲和色谱法：模型研究证明了该技术在机理研究以及多底物酶纯化方面的潜力。
FEBS Lett. 1972 Apr 1;21(3):281-285. doi: 10.1016/0014-5793(72)80183-2.
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Affinity chromatography of enzymes on insolubilized cofactors.
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Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides.通过卤化氰将肽和蛋白质与多糖进行化学偶联。
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Purification of dihydrofolic reductase from chicken liver by affinity chromatography.通过亲和层析法从鸡肝中纯化二氢叶酸还原酶。
Biochem Biophys Res Commun. 1971 Aug 6;44(3):608-13. doi: 10.1016/s0006-291x(71)80126-2.
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Affinity chromatography of transaminases.
Anal Biochem. 1971 Jul;42(1):48-53. doi: 10.1016/0003-2697(71)90008-x.
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Interactions between an unusual aspartate aminotransferase from Rhizobium japonicum and pyridoxal-5'-phosphate studied by affinity chromatography.
FEBS Lett. 1972 Jun 1;23(1):73-6. doi: 10.1016/0014-5793(72)80288-6.
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Selective enzyme purification by affinity chromatography.通过亲和色谱法进行选择性酶纯化。
Proc Natl Acad Sci U S A. 1968 Oct;61(2):636-43. doi: 10.1073/pnas.61.2.636.
10
Some applications of insolubilised cofactors to the purification of pyridine nucleotide-dependent dehydrogenases.不溶性辅因子在吡啶核苷酸依赖性脱氢酶纯化中的一些应用。
Biochem Biophys Res Commun. 1972 Aug 21;48(4):1004-10. doi: 10.1016/0006-291x(72)90708-5.