大肠杆菌K12的5'-肌苷酸脱氢酶：5'-鸟苷酸的抑制性质
Inosine 5'-monophosphate dehydrogenase of Escherichia coli K12: the nature of the inhibition by guanosine 5'-monophosphate.
作者信息
Lambden P R, Drabble W T
出版信息
Biochem J. 1973 Jul;133(3):607-8. doi: 10.1042/bj1330607.
Abstract
When IMP is the variable substrate, IMP dehydrogenase (EC 1.2.1.14) gives non-linear Lineweaver-Burk plots in the presence of GMP. At 0.1mm-GMP the Hill coefficient n=1.15 and at 0.2mm-GMP n=1.34. In the absence of GMP n=1.0. The fact that n exceeds 1.0 in the presence of GMP indicates that the enzyme possesses regulatory (allosteric) properties. The inhibition by GMP is competitive with respect to IMP and non-competitive with respect to NAD(+).
摘要
当肌苷酸（IMP）作为可变底物时，在鸟苷酸（GMP）存在的情况下，IMP脱氢酶（EC 1.2.1.14）给出非线性的林-贝氏图。在0.1mM - GMP时，希尔系数n = 1.15，在0.2mM - GMP时，n = 1.34。在没有GMP的情况下，n = 1.0。在GMP存在时n超过1.0这一事实表明该酶具有调节（别构）特性。GMP的抑制作用相对于IMP是竞争性的，相对于烟酰胺腺嘌呤二核苷酸（NAD(+)）是非竞争性的。
相似文献
1
Inosine 5'-monophosphate dehydrogenase of Escherichia coli K12: the nature of the inhibition by guanosine 5'-monophosphate.大肠杆菌K12的5'-肌苷酸脱氢酶：5'-鸟苷酸的抑制性质
Biochem J. 1973 Jul;133(3):607-8. doi: 10.1042/bj1330607.
2
Affinity chromatography on immobilised nucleotides. The synthesis, specificity and applications of immobilised inosine 5'-monophosphate.固定化核苷酸亲和层析。固定化5'-肌苷单磷酸的合成、特异性及应用。
Eur J Biochem. 1980 Sep;110(1):279-88. doi: 10.1111/j.1432-1033.1980.tb04866.x.
3
Inosine 5'-monophosphate dehydrogenase of Escherichia coli. Purification by affinity chromatography, subunit structure and inhibition by guanosine 5'-monophosphate.大肠杆菌的肌苷5'-单磷酸脱氢酶。通过亲和色谱法纯化、亚基结构及受鸟苷5'-单磷酸的抑制作用
Biochem J. 1979 Dec 1;183(3):481-94. doi: 10.1042/bj1830481.
4
Inhibitors of inosinic acid dehydrogenase. 2-Substituted inosinic acids.肌苷酸脱氢酶抑制剂。2-取代肌苷酸
J Med Chem. 1984 Apr;27(4):429-32. doi: 10.1021/jm00370a003.
5
Active-site modification of native and mutant forms of inosine 5'-monophosphate dehydrogenase from Escherichia coli K12.来自大肠杆菌K12的肌苷5'-单磷酸脱氢酶天然形式和突变形式的活性位点修饰
Biochem J. 1980 Nov 1;191(2):533-41. doi: 10.1042/bj1910533.
6
Inosinic acid 5'-monophosphate dehydrogenase of Escherichia coli: purification by affinity chromatography and some properties.大肠杆菌的肌苷酸5'-单磷酸脱氢酶：通过亲和层析法进行纯化及某些特性研究
Arch Biochem Biophys. 1975 Oct;170(2):567-75. doi: 10.1016/0003-9861(75)90152-6.
7
Human IMP dehydrogenase. Kinetics and regulatory properties.人肌苷酸脱氢酶。动力学和调节特性。
Biochim Biophys Acta. 1974 Oct 17;364(2):209-17. doi: 10.1016/0005-2744(74)90006-0.
8
Studies on inosine monophosphate dehydrogenase. Steady state kinetics.肌苷单磷酸脱氢酶的研究。稳态动力学。
Biochim Biophys Acta. 1976 May 13;429(3):645-60. doi: 10.1016/0005-2744(76)90314-4.
9
Purification and properties of inosine monophosphate oxidoreductase from nitrogen-fixing nodules of cowpea (Vigna unguiculata L. Walp).豇豆（Vigna unguiculata L. Walp）固氮根瘤中肌苷单磷酸氧化还原酶的纯化及性质
Arch Biochem Biophys. 1985 Feb 1;236(2):807-14. doi: 10.1016/0003-9861(85)90687-3.
10
Mechanism of action of 1- -D-ribofuranosyl-1,2,4-triazole-3-carboxamide (Virazole), a new broad-spectrum antiviral agent.新型广谱抗病毒药物1-β-D-呋喃核糖基-1,2,4-三唑-3-甲酰胺（病毒唑）的作用机制
Proc Natl Acad Sci U S A. 1973 Apr;70(4):1174-8. doi: 10.1073/pnas.70.4.1174.
引用本文的文献
1
A mathematical model for the adenylosuccinate synthetase reaction involved in purine biosynthesis.一种参与嘌呤生物合成的腺苷酸琥珀酸合成酶反应的数学模型。
Theor Biol Med Model. 2007 Feb 27;4:11. doi: 10.1186/1742-4682-4-11.
2
Inosine 5'-monophosphate dehydrogenase of Escherichia coli. Purification by affinity chromatography, subunit structure and inhibition by guanosine 5'-monophosphate.大肠杆菌的肌苷5'-单磷酸脱氢酶。通过亲和色谱法纯化、亚基结构及受鸟苷5'-单磷酸的抑制作用
Biochem J. 1979 Dec 1;183(3):481-94. doi: 10.1042/bj1830481.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Guanosine 5'-phosphate reductase and its role in the interconversion of purine nucleotides.鸟苷 5'-磷酸还原酶及其在嘌呤核苷酸相互转化中的作用。
J Biol Chem. 1960 May;235:1474-8.
3
4
5
Inosine-5'-phosphate dehydrogenase of Aerobacter aerogenes. Studies of tertiary structure.
Biochim Biophys Acta. 1970 May 13;206(2):215-23. doi: 10.1016/0005-2744(70)90105-1.
6
Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5'-phosphate dehydrogenase, xanthosine-5'-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis.
J Biochem. 1968 May;63(5):661-9. doi: 10.1093/oxfordjournals.jbchem.a128826.
7
Demonstration of an effector site for the enzyme inosine 5'-phosphate dehydrogenase.肌苷5'-磷酸脱氢酶的酶效应物位点的证明。
Biochem Biophys Res Commun. 1968 Mar 27;30(6):673-7. doi: 10.1016/0006-291x(68)90565-2.
8
Rajagopalan KV, Handler P: Purification and properties of inosinic acid dehydrogenase from Escherichia coli.
J Biol Chem. 1969 Sep 10;244(17):4793-7.
9
Inosine 5'-phosphate dehydrogenase. Site of inhibition by guanosine 5'-phosphate and of inactivation by 6-chloro- and 6-mercaptopurine ribonucleoside 5'-phosphates.
Biochemistry. 1967 Mar;6(3):679-89. doi: 10.1021/bi00855a006.
Zotero 插件