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大肠杆菌K12的5'-肌苷酸脱氢酶：5'-鸟苷酸的抑制性质

Inosine 5'-monophosphate dehydrogenase of Escherichia coli K12: the nature of the inhibition by guanosine 5'-monophosphate.

作者信息

Lambden P R, Drabble W T

出版信息

Biochem J. 1973 Jul;133(3):607-8. doi: 10.1042/bj1330607.

DOI:10.1042/bj1330607
PMID:4354743
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1177741/
Abstract

When IMP is the variable substrate, IMP dehydrogenase (EC 1.2.1.14) gives non-linear Lineweaver-Burk plots in the presence of GMP. At 0.1mm-GMP the Hill coefficient n=1.15 and at 0.2mm-GMP n=1.34. In the absence of GMP n=1.0. The fact that n exceeds 1.0 in the presence of GMP indicates that the enzyme possesses regulatory (allosteric) properties. The inhibition by GMP is competitive with respect to IMP and non-competitive with respect to NAD(+).

摘要

当肌苷酸（IMP）作为可变底物时，在鸟苷酸（GMP）存在的情况下，IMP脱氢酶（EC 1.2.1.14）给出非线性的林-贝氏图。在0.1mM - GMP时，希尔系数n = 1.15，在0.2mM - GMP时，n = 1.34。在没有GMP的情况下，n = 1.0。在GMP存在时n超过1.0这一事实表明该酶具有调节（别构）特性。GMP的抑制作用相对于IMP是竞争性的，相对于烟酰胺腺嘌呤二核苷酸（NAD(+)）是非竞争性的。

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本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Guanosine 5'-phosphate reductase and its role in the interconversion of purine nucleotides.鸟苷 5'-磷酸还原酶及其在嘌呤核苷酸相互转化中的作用。
J Biol Chem. 1960 May;235:1474-8.
3
KINETICS OF REGULATORY ENZYMES. KINETIC ORDER OF THE YEAST DIPHOSPHOPYRIDINE NUCLEOTIDE ISOCITRATE DEHYDROGENASE REACTION AND A MODEL FOR THE REACTION.调节酶的动力学。酵母二磷酸吡啶核苷酸异柠檬酸脱氢酶反应的动力学级数及反应模型。
J Biol Chem. 1965 Jun;240:2682-90.
4
Enzymes essential for the biosynthesis of nucleic acid guanine; inosine 5'-phosphate dehydrogenase of Aerobacter aerogenes.核酸鸟嘌呤生物合成所必需的酶；产气气杆菌的肌苷5'-磷酸脱氢酶。
J Biol Chem. 1957 May;226(1):339-50.
5
Inosine-5'-phosphate dehydrogenase of Aerobacter aerogenes. Studies of tertiary structure.
Biochim Biophys Acta. 1970 May 13;206(2):215-23. doi: 10.1016/0005-2744(70)90105-1.
6
Regulation of purine ribonucleotide synthesis by end product inhibition. I. Effect of purine nucleotides on inosine-5'-phosphate dehydrogenase, xanthosine-5'-phosphate aminase and adenylosuccinate lyase of Bacillus subtilis.
J Biochem. 1968 May;63(5):661-9. doi: 10.1093/oxfordjournals.jbchem.a128826.
7
Demonstration of an effector site for the enzyme inosine 5'-phosphate dehydrogenase.肌苷5'-磷酸脱氢酶的酶效应物位点的证明。
Biochem Biophys Res Commun. 1968 Mar 27;30(6):673-7. doi: 10.1016/0006-291x(68)90565-2.
8
Rajagopalan KV, Handler P: Purification and properties of inosinic acid dehydrogenase from Escherichia coli.
J Biol Chem. 1969 Sep 10;244(17):4793-7.
9
Inosine 5'-phosphate dehydrogenase. Site of inhibition by guanosine 5'-phosphate and of inactivation by 6-chloro- and 6-mercaptopurine ribonucleoside 5'-phosphates.
Biochemistry. 1967 Mar;6(3):679-89. doi: 10.1021/bi00855a006.