大肠杆菌K12 crp基因的克隆与序列分析
Cloning and sequence of the crp gene of Escherichia coli K 12.
作者信息
Cossart P, Gicquel-Sanzey B
出版信息
Nucleic Acids Res. 1982 Feb 25;10(4):1363-78. doi: 10.1093/nar/10.4.1363.
Abstract
We have determined the nucleotide sequence of the crp gene of Escherichia coli K 12. From a lambda transducing phage, the crp region was subcloned into pBR322. The gene was localized on the cloned fragment by determining the length of deletions which affect its expression. Its nucleotide sequence was established by using the technique of Maxam and Gilbert. The deduced amino-acid sequence is in agreement with the previously published amino acid composition of the protein (1, 2). Analysis of the sequence confirms that the DNA binding domain is located in the C-terminal portion of the protein.
摘要
我们已经测定了大肠杆菌K12的crp基因的核苷酸序列。从一个λ转导噬菌体中，将crp区域亚克隆到pBR322中。通过确定影响其表达的缺失片段的长度，将该基因定位在克隆片段上。利用马克萨姆和吉尔伯特技术确定了其核苷酸序列。推导的氨基酸序列与先前发表的该蛋白质的氨基酸组成一致(1,2)。序列分析证实，DNA结合结构域位于该蛋白质的C末端部分。
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