乳糖阻遏蛋白与特定核苷酸序列的合成DNA的结合。
Lac repressor binding to synthetic DNAs of defined nucleotide sequence.
作者信息
Riggs A D, Lin S, Wells R D
出版信息
Proc Natl Acad Sci U S A. 1972 Mar;69(3):761-4. doi: 10.1073/pnas.69.3.761.
Abstract
Binding of lac repressor to 20 synthetic DNAs of high molecular weight with defined repeating sequences was investigated by competition experiments. Although none of these DNAs binds repressor as tightly as does lac operator, most do bind to a measurable extent. Their affinity for repressor varies greatly and is a function of both nucleotide composition and sequence. Poly(dC-dC).poly(dG-dC) competes for repressor 200-times less well than either poly(dA-dT).poly(dA-dT) or poly(dT-dT-dG).poly(dC-dA-dA). The other DNAs show a broad spectrum of affinities for repressor between these extremes. These results show that the lac repressor has affinity for, and can distinguish between, sequences distantly related to its operator.
摘要
通过竞争实验研究了乳糖阻遏物与20种具有明确重复序列的高分子量合成DNA的结合情况。尽管这些DNA中没有一种像乳糖操纵基因那样紧密地结合阻遏物，但大多数确实能在一定程度上结合。它们对阻遏物的亲和力差异很大，并且是核苷酸组成和序列的函数。聚（dC-dC）·聚（dG-dC）与阻遏物竞争的能力比聚（dA-dT）·聚（dA-dT）或聚（dT-dT-dG）·聚（dC-dA-dA）低200倍。其他DNA在这些极端情况之间对阻遏物表现出广泛的亲和力谱。这些结果表明，乳糖阻遏物对与其操纵基因远距离相关的序列具有亲和力，并且能够区分它们。
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