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[用铁蛋白抗体技术对鸡肌原纤维中腺嘌呤成分的定位]

[Localization of adenine components in chick myofibrils by the ferritin antibody technic].

作者信息

Ogievetskaia M M, Podgorsdnichenko V K, Samosudova N V

出版信息

Biofizika. 1979 Jan-Feb;24(1):101-3.

PMID:435520
Abstract

The immunochemical method has been proposed to indicate the adenin compounds in isolated myofibrils of striated muscle. Adenosine--containing antigen was prepared by coupling adenosine with bovine serum albumin (adenosine--BSA). Antibodies against adenosine--BSA were labelled by ferritin. The sites of the antigen antibody reaction were marked by localization of ferritin molecules. Ferritin antibodies were found to concentrate in the following sarcomere sites: M and Z bands, N-lines and terminal cisternae of sarcoplasmic reticulum near the Z-bands.

摘要

有人提出用免疫化学方法来检测横纹肌分离肌原纤维中的腺嘌呤化合物。通过将腺苷与牛血清白蛋白偶联制备含腺苷的抗原(腺苷 - 牛血清白蛋白)。抗腺苷 - 牛血清白蛋白的抗体用铁蛋白标记。抗原抗体反应的位点通过铁蛋白分子的定位来标记。发现铁蛋白抗体集中在以下肌节部位:M带和Z带、N线以及靠近Z带的肌质网终末池。

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