Richardson F L, Stromer M H, Huiatt T W, Robson R M
Eur J Cell Biol. 1981 Dec;26(1):91-101.
Antisera or affinity-purified antibodies shown to be specific for avian gizzard desmin antigen by double immunodiffusion, antigen-blocking, and immunoautoradiography experiments have been used in indirect immunofluorescence and indirect immunoelectron microscopy to demonstrate localization of desmin in myofibrils from mature avian muscles. The light microscope results agree with the work of others in that they suggest that desmin is primarily at or near the periphery of Z-lines of striated muscle myofibrils. Immunoperoxidase labelling more clearly shows that the reactive desmin antigen is located almost entirely between Z-lines of adjacent parallel myofibrils and that there is no obvious correlation between locations of T-tubules and desmin structures. The electron-dense reaction product often followed an approximately linear course between Z-lines of adjacent myofibrils and indicated the desmin antibodies had decorated a small number of filaments spanning this region. These results suggest that the desmin found in close association with myofibrils of mature striated muscle is in the aggregated form of 10-nm filaments.
通过双向免疫扩散、抗原阻断和免疫放射自显影实验证明对禽肌胃结蛋白抗原具有特异性的抗血清或亲和纯化抗体，已用于间接免疫荧光和间接免疫电子显微镜，以证明结蛋白在成熟禽肌肉肌原纤维中的定位。光学显微镜结果与其他人的研究一致，表明结蛋白主要位于横纹肌肌原纤维Z线的周边或附近。免疫过氧化物酶标记更清楚地显示，有反应性的结蛋白抗原几乎完全位于相邻平行肌原纤维的Z线之间，并且T小管的位置与结蛋白结构之间没有明显的相关性。电子致密反应产物通常在相邻肌原纤维的Z线之间呈大致线性分布，表明结蛋白抗体标记了跨越该区域的少量细丝。这些结果表明，在成熟横纹肌肌原纤维紧密结合处发现的结蛋白是10纳米细丝的聚集形式。
