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[利用脉冲核磁共振技术测量生物系统中水分子的磁弛豫并计算其体积]

[Measurement of the magnetic relaxation of water molecules in biological systems by the impulse NMR technic with calculation of their volume].

作者信息

Anisimov A V, Miftakhutdinova F G

出版信息

Biofizika. 1979 Jan-Feb;24(1):113-6.

PMID:435522
Abstract

Impulse sequences are presented for selective by biological system volume measurements of the times of spin--spin, spin--lattice relaxation of water molecules by means of NMR spin echo. Selectivity of the programs is based on the fact that in the presence of magnetic field impulse gradient diffusion quenching of the echo differs for water molecules limited or unlimited in their diffusion displacement by some barriers (membranes) and it also differs for molecules limited in compartments of different size. Selective measurement of the times of spin--spin relaxation (T2) of water in intracellular structures of the yeast cells gave the value 24.4 msec, which is 1.3 times lower than the specimen volume measured by standard method.

摘要

通过核磁共振自旋回波,给出了用于通过生物系统体积测量水分子自旋 - 自旋、自旋 - 晶格弛豫时间的脉冲序列。这些程序的选择性基于这样一个事实:在存在磁场脉冲梯度的情况下,对于在扩散位移上受到某些屏障(膜)限制或不受限制的水分子,回波的扩散猝灭是不同的,并且对于限制在不同大小隔室中的分子也是不同的。对酵母细胞内结构中水的自旋 - 自旋弛豫时间(T2)进行选择性测量,得到的值为24.4毫秒,该值比用标准方法测量的样品体积低1.3倍。

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