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[通过核磁共振脉冲法对冻干的浅白隐球酵母变种易流酵母细胞中隔离水的平移流动性研究]

[Translation mobility of water isolated in the cells of lyophilized Cryptococcus albidus var. diffluens yeasts by the NMR impulse method].

作者信息

Anisimov A V, Miftakhutdinova F G, Aksenov S I

出版信息

Biofizika. 1978 May-Jun;23(3):479-84.

PMID:667152
Abstract

The method of NMR spin echo in a combination with the impulse gradient of the magnetic field was used to study the self-diffusion of water isolated in the cells of lyophylized Criptococcus. A relative fall of the amplitude of spin echo (factor R) in relation to the value of magnetic field impulse gradient (g), their duration (delta) and temporary distance between them (delta). The studies were carried out in the temperature range of 20 degrees divided by 80 degrees C. It has been shown that the fraction of isolated mobile water is located in intracellular permeable compartments with an average size of 0.6.10--4 cm at 20 degrees C. The coefficient of selfdiffusion of isolated water of Criptococcus (0.25.10--5 cm2S--1 at 20 degrees C) and the activation energy of selfdiffusion (4.4 ccal/mole at 20 degrees divided by 60 degrees C) were determined, permeability of walls of water-containing compartments taken into account.

摘要

采用核磁共振自旋回波法结合磁场脉冲梯度来研究冻干隐球菌细胞中隔离水的自扩散。自旋回波幅度的相对下降(因子R)与磁场脉冲梯度值(g)、其持续时间(δ)以及它们之间的时间间隔(δ)有关。研究在20℃至80℃的温度范围内进行。结果表明,在20℃时,隔离的流动水部分位于平均尺寸为0.6×10⁻⁴厘米的细胞内可渗透隔室中。确定了隐球菌隔离水的自扩散系数(20℃时为0.25×10⁻⁵厘米²/秒)和自扩散活化能(20℃至60℃时为4.4千卡/摩尔),同时考虑了含水隔室壁的渗透性。

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