Goldstein I, Hoffstein S, Gallin J, Weissmann G
Proc Natl Acad Sci U S A. 1973 Oct;70(10):2916-20. doi: 10.1073/pnas.70.10.2916.
A low-molecular-weight component of complement, similar to or identical with human C5a, interacts with human polymorphonuclear leukocytes treated with cytochalasin B and provokes extracellular release of lysosomal enzymes from these cells. Enzyme release occurs in the absence of particles and is selective in that it is not accompained by release of cytoplasmic enzymes. Cell viability is not altered. Pharmacologic agents that regulate secretion of other inflammatory mediators influenced complement-dependent enzyme release: cAMP and theophylline, prostaglandin E(1) and colchicine inhibited, whereas cGMP enhanced release of enzymes. Ultra-structural histochemistry of cells exposed to this component of complement revealed degranulation, fusion of lysosomal with plasma membranes, and transient assembly of microtubules associated with the release of endogenous myeloperoxidase. Our findings suggest that these intracellular events are common to two important responses of polymorphonuclear leukocytes in inflammation and tissue injury: (a) release of lysosomal hydrolases and (b) chemotaxis.
一种与人类C5a相似或相同的补体低分子量成分，与用细胞松弛素B处理的人类多形核白细胞相互作用，并促使这些细胞从细胞外释放溶酶体酶。酶的释放发生在没有颗粒的情况下，并且具有选择性，因为它不会伴随着细胞质酶的释放。细胞活力没有改变。调节其他炎症介质分泌的药物会影响补体依赖性酶的释放：环磷酸腺苷（cAMP）和茶碱、前列腺素E(1)和秋水仙碱会抑制酶的释放，而环磷酸鸟苷（cGMP）则会增强酶的释放。对暴露于这种补体成分的细胞进行超微结构组织化学分析，结果显示有脱颗粒现象、溶酶体与质膜融合以及与内源性髓过氧化物酶释放相关的微管瞬时组装。我们的研究结果表明，这些细胞内事件是多形核白细胞在炎症和组织损伤中的两种重要反应所共有的：（a）溶酶体水解酶的释放和（b）趋化性。
