直接测量质子与猪心乳酸脱氢酶活性三元复合物的结合。
Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase.
作者信息
Holbrook J J
出版信息
Biochem J. 1973 Aug;133(4):847-9. doi: 10.1042/bj1330847.
Abstract
With the use of a glass electrode it is shown that one proton is taken up from the solvent when o-nitrophenylpyruvate forms a ternary complex with lactate dehydrogenase and NADH at pH8 and before the complex has rearranged to give products.
摘要
使用玻璃电极表明，当邻硝基苯丙酮酸在pH8时与乳酸脱氢酶和NADH形成三元复合物且在复合物重排产生产物之前，一个质子从溶剂中被摄取。
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Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase.直接测量质子与猪心乳酸脱氢酶活性三元复合物的结合。
Biochem J. 1973 Aug;133(4):847-9. doi: 10.1042/bj1330847.
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Factors controlling the interconversion of enzyme-substrate compounds of pig heart lactate dehydrogenase.控制猪心乳酸脱氢酶酶 - 底物化合物相互转化的因素。
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本文引用的文献
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Approaches to the study of enzyme mechanisms lactate dehydrogenase.乳酸脱氢酶的酶机制研究方法
FEBS Lett. 1973 Apr 15;31(2):157-169. doi: 10.1016/0014-5793(73)80095-x.
2
The use of ternary complexes to study ionizations and isomerizations during catalysis by lactate dehydrogenase.利用三元复合物研究乳酸脱氢酶催化过程中的电离和异构化。
Biochem J. 1973 Apr;131(4):739-48. doi: 10.1042/bj1310739.
3
Ionic properties of an essential histidine residue in pig heart lactate dehydrogenase.猪心乳酸脱氢酶中一个必需组氨酸残基的离子特性
Biochem J. 1973 Apr;131(4):729-38. doi: 10.1042/bj1310729.
4
Equilibrium binding of nicotinamide nucleotides to lactate dehydrogenases.烟酰胺核苷酸与乳酸脱氢酶的平衡结合
Biochem J. 1973 Apr;131(4):719-28. doi: 10.1042/bj1310719.
5
Structural constraints of possible mechanisms of lactate dehydrogenase as shown by high resolution studies of the apoenzyme and a variety of enzyme complexes.通过对脱辅酶和多种酶复合物的高分辨率研究显示的乳酸脱氢酶可能机制的结构限制。
Cold Spring Harb Symp Quant Biol. 1972;36:179-91. doi: 10.1101/sqb.1972.036.01.025.
6
[Labelling of essential amino acid residues of pig heart lactate dehydrogenase with (carbonyl-14C)3-(2-bromoacetyl) pyridine].[用（羰基 - ¹⁴C）₃ - （2 - 溴乙酰基）吡啶对猪心乳酸脱氢酶的必需氨基酸残基进行标记]
Hoppe Seylers Z Physiol Chem. 1969 Apr;350(4):473-83.
7
Lactic dehydrogenase. X. A re-evaluation of the effects of pH upon the kinetics of the reaction.
J Biol Chem. 1967 Jul 25;242(14):3245-52.
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