乳酸脱氢酶与其底物反应某些步骤的解析。
The resolution of some steps of the reactions of lactate dehydrogenase with its substrates.
作者信息
Heck H D, McMurray C H, Gutfreund H
出版信息
Biochem J. 1968 Aug;108(5):793-6. doi: 10.1042/bj1080793.
Abstract
- The reaction of pig heart lactate dehydrogenase (EC 1.1.1.27) with NAD(+) and lactate to form pyruvate and NADH was followed by rapid spectrophotometric methods. The distinct spectrum of enzyme-bound NADH permits the measurement of the rate of dissociation of this compound. 2. The reduction of the first mole equivalent of NAD(+) per mole of enzyme sites can also be observed, and is much more rapid than the steady-state rate of NADH production. 3. At pH8 the dissociation of the enzyme-NADH complex is rate-determining for the steady-state oxidation of lactate. At lower pH some other step after the interconversion of the ternary complex and before the dissociation of NADH is rate-determining. Other evidence for a compulsory-order mechanism is provided.
摘要
- 采用快速分光光度法跟踪猪心乳酸脱氢酶（EC 1.1.1.27）与NAD⁺和乳酸反应生成丙酮酸和NADH的过程。酶结合的NADH独特的光谱使得能够测量该化合物的解离速率。2. 还可以观察到每摩尔酶位点上第一摩尔当量的NAD⁺的还原，其比NADH产生的稳态速率快得多。3. 在pH8时，酶 - NADH复合物的解离是乳酸稳态氧化的速率决定步骤。在较低pH值下，三元复合物相互转化后且NADH解离之前的其他步骤是速率决定步骤。还提供了强制顺序机制的其他证据。
相似文献
1
The resolution of some steps of the reactions of lactate dehydrogenase with its substrates.乳酸脱氢酶与其底物反应某些步骤的解析。
Biochem J. 1968 Aug;108(5):793-6. doi: 10.1042/bj1080793.
2
The identification of intermediates in the reaction of pig heart lactate dehydrogenase with its substrates.猪心乳酸脱氢酶与其底物反应中间体的鉴定。
Biochem J. 1974 Jun;139(3):677-97. doi: 10.1042/bj1390677.
3
Pig heart lactate dehydrogenase. Binding of pyruvate and the interconversion of pyruvate-containing ternary complexes.猪心脏乳酸脱氢酶。丙酮酸的结合以及含丙酮酸三元复合物的相互转化。
Biochem J. 1975 Dec;151(3):715-27. doi: 10.1042/bj1510715.
4
The use of ternary complexes to study ionizations and isomerizations during catalysis by lactate dehydrogenase.利用三元复合物研究乳酸脱氢酶催化过程中的电离和异构化。
Biochem J. 1973 Apr;131(4):739-48. doi: 10.1042/bj1310739.
5
Transient-kinetic studies of pig muscle lactate dehydrogenase.猪肌肉乳酸脱氢酶的瞬态动力学研究。
Biochem J. 1971 Jan;121(2):235-40. doi: 10.1042/bj1210235.
6
The kinetics of the interconversion of intermediates of the reaction of pig muscle lactate dehydrogenase with oxidized nicotinamide-adenine dinucleotide and lactate.猪肌肉乳酸脱氢酶与氧化型烟酰胺腺嘌呤二核苷酸及乳酸反应中间体相互转化的动力学
Biochem J. 1973 Sep;135(1):81-5. doi: 10.1042/bj1350081.
7
Malate dehydrogenase of the cytosol. A kinetic investigation of the reaction mechanism and a comparison with lactate dehydrogenase.胞质苹果酸脱氢酶。反应机制的动力学研究及与乳酸脱氢酶的比较。
Biochem J. 1978 Dec 1;175(3):987-98. doi: 10.1042/bj1750987.
8
The lactate dehydrogenase--reduced nicotinamide--adenine dinucleotide--pyruvate complex. Kinetics of pyruvate binding and quenching of coeznyme fluorescence.乳酸脱氢酶-还原型烟酰胺-腺嘌呤二核苷酸-丙酮酸复合物。丙酮酸结合动力学及辅酶荧光猝灭。
Biochem J. 1974 Apr;139(1):251-9. doi: 10.1042/bj1390251.
9
Direct measurement of proton binding to the active ternary complex of pig heart lactate dehydrogenase.直接测量质子与猪心乳酸脱氢酶活性三元复合物的结合。
Biochem J. 1973 Aug;133(4):847-9. doi: 10.1042/bj1330847.
10
Ternary complex formation of pig heart lactate dehydrogenase with spin-labelled coenzyme and inhibitors as studied by electron spin resonance.通过电子自旋共振研究猪心乳酸脱氢酶与自旋标记辅酶及抑制剂的三元复合物形成。
Biochim Biophys Acta. 1979 Jun 6;568(2):287-96. doi: 10.1016/0005-2744(79)90296-1.
引用本文的文献
1
Mechanistic Analysis of Fluorescence Quenching of Reduced Nicotinamide Adenine Dinucleotide by Oxamate in Lactate Dehydrogenase Ternary Complexes.关于草酰氨在乳酸脱氢酶三元复合物中使还原型烟酰胺腺嘌呤二核苷酸荧光猝灭的机制分析。
Photochem Photobiol. 2017 Oct;93(5):1193-1203. doi: 10.1111/php.12775. Epub 2017 Jun 22.
2
Active-Loop Dynamics within the Michaelis Complex of Lactate Dehydrogenase from Bacillus stearothermophilus.嗜热脂肪芽孢杆菌乳酸脱氢酶米氏复合物中的活性环动力学
Biochemistry. 2016 Jul 12;55(27):3803-14. doi: 10.1021/acs.biochem.6b00091. Epub 2016 Jun 30.
3
The identification of intermediates in the reaction of pig heart lactate dehydrogenase with its substrates.猪心乳酸脱氢酶与其底物反应中间体的鉴定。
Biochem J. 1974 Jun;139(3):677-97. doi: 10.1042/bj1390677.
4
The kinetics of the interconversion of intermediates of the reaction of pig muscle lactate dehydrogenase with oxidized nicotinamide-adenine dinucleotide and lactate.猪肌肉乳酸脱氢酶与氧化型烟酰胺腺嘌呤二核苷酸及乳酸反应中间体相互转化的动力学
Biochem J. 1973 Sep;135(1):81-5. doi: 10.1042/bj1350081.
5
Rate-determining processes and the number of simultaneously active sties of D-glyceraldehyde 3-phosphate dehydrogenase.3-磷酸甘油醛脱氢酶的限速过程及同时活跃位点的数量
Biochem J. 1971 Mar;122(1):71-7. doi: 10.1042/bj1220071.
6
Transient-kinetic studies of pig muscle lactate dehydrogenase.猪肌肉乳酸脱氢酶的瞬态动力学研究。
Biochem J. 1971 Jan;121(2):235-40. doi: 10.1042/bj1210235.
7
Reactivity of the essential thiol group of lactate dehydrogenase and substrate binding.乳酸脱氢酶必需巯基的反应性与底物结合
Biochem J. 1970 Nov;120(2):289-97. doi: 10.1042/bj1200289.
8
[Structural flexibility and enzyme function].
Naturwissenschaften. 1969 May;56(5):232-7. doi: 10.1007/BF00633916.
9
Structural adaptations of lactate dehydrogenase isozymes.乳酸脱氢酶同工酶的结构适应性
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2677-81. doi: 10.1073/pnas.74.7.2677.
本文引用的文献
1
2
[Effects of sulfite on diphosphopyridine nucleotide hydrolizing enzymes].[亚硫酸盐对二磷酸吡啶核苷酸水解酶的影响]
Biochem Z. 1956;328(3):187-94.
3
4
Optical and chemical identification of kinetic steps in trypsin- and chymotrypsin-catalysed reactions.胰蛋白酶和胰凝乳蛋白酶催化反应中动力学步骤的光学与化学鉴定。
Biochem J. 1966 Nov;101(2):411-6. doi: 10.1042/bj1010411.
5
[Regulation of the activity of glutamate dehydrogenase by effectors GTP and ADP: study by means of "stopped flow"].[效应物GTP和ADP对谷氨酸脱氢酶活性的调节：采用“停流法”的研究]
Bull Soc Chim Biol (Paris). 1967 Dec 18;49(11):1563-72.
6
The kinetics of the reversible inhibition of heart lactate dehydrogenase through the formation of the enzyme-oxidized nicotinamide-adenine dinucleotide-pyruvate compounds.通过形成酶-氧化型烟酰胺腺嘌呤二核苷酸-丙酮酸化合物对心脏乳酸脱氢酶进行可逆抑制的动力学。
Biochem J. 1968 Feb;106(3):683-7. doi: 10.1042/bj1060683.
7
The reaction of liver alcohol dehydrogenase with reduced diphosphopyridine nucleotide.
J Biol Chem. 1967 Sep 25;242(18):4275-8.
8
9
Lactic dehydrogenase. X. A re-evaluation of the effects of pH upon the kinetics of the reaction.
J Biol Chem. 1967 Jul 25;242(14):3245-52.
Zotero 插件