Ide P R, Stevenson R G
Can J Comp Med. 1973 Oct;37(4):347-55.
A flock of four week old chickens experienced a disease of sudden onset in which the only symptoms were those of depression shortly before death, and in which the predominant histological lesion was necrosis of lymphocytes in the bursa of Fabricius.A virus, designated strain Sk-1, was isolated from pooled bursal tissue of affected birds and was serologically identified as a strain of the infectious bursal agent. This virus was chloroform resistant, did not hemagglutinate guinea pig or chicken erythrocytes and did not produce a cytopathic effect in chick embryo tissue cultures. Equivocal results were obtained in filtration studies but the agent was less than 100nm in diameter. Four week old chicks inoculated with strain Sk-1 developed microscopic lesions in the bursa of Fabricius which were similar to those seen in the original field specimens. Inoculated chick embryos exhibited characteristic macroscopic lesions and necrosis of vascular tissue was a common histological change.A limited serological survey of local poultry flocks indicated that infection by this agent had occurred in four of the ten flocks examined.
一群4周龄的鸡突然发病，死前唯一症状是精神沉郁，主要组织学病变是法氏囊中淋巴细胞坏死。从患病鸡的法氏囊组织中分离出一种病毒，命名为Sk-1株，血清学鉴定为传染性法氏囊病病原体毒株。该病毒对氯仿有抗性，不凝集豚鼠或鸡红细胞，在鸡胚组织培养中不产生细胞病变效应。过滤研究结果不明确，但该病原体直径小于100nm。接种Sk-1株的4周龄雏鸡在法氏囊中出现微观病变，与最初现场样本中的病变相似。接种的鸡胚表现出特征性宏观病变，血管组织坏死是常见的组织学变化。对当地家禽群的有限血清学调查表明，在所检查的10个鸡群中有4个发生了该病原体感染。
