Studies on polynucleotides containing hybrid sequences. Synthesis of oligonucleotides containing thymidine, adenosine, and a single deoxyribonucleotidyl-(3'-5')-ribonucleotide linkage.
作者信息
McCutchan T F, Gilham P T
出版信息
Biochemistry. 1973 Nov 20;12(24):4840-6. doi: 10.1021/bi00748a005.
PMID:4357549
Abstract
摘要
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Studies on polynucleotides containing hybrid sequences. Synthesis of oligonucleotides containing thymidine, adenosine, and a single deoxyribonucleotidyl-(3'-5')-ribonucleotide linkage.含杂交序列的多核苷酸研究。含胸苷、腺苷及单个脱氧核糖核苷酸基 -（3'-5'）-核糖核苷酸连接的寡核苷酸的合成。
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'Single addition' and 'transnucleotidation' reactions catalyzed by polynucleotide phosphorylase. Effect of enzymatic removal of inorganic phosphate during reaction.多核苷酸磷酸化酶催化的“单加成”和“转核苷酸作用”反应。反应过程中酶促去除无机磷酸盐的影响。
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Modification of end phosphate gruops in mono- and oligonucleotides.单核苷酸和寡核苷酸末端磷酸基团的修饰。
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