单核苷酸和寡核苷酸末端磷酸基团的修饰。
Modification of end phosphate gruops in mono- and oligonucleotides.
作者信息
Shumyantzeva V V, Sokolova N I, Shabarova Z A
出版信息
Nucleic Acids Res. 1976 Apr;3(4):903-16. doi: 10.1093/nar/3.4.903.
A method is described for selective activation of phosphomonoester end groups of oligonucleotides and nucleosidedi-(tri) phosphates via mixed anhydrides with mesitoic acid. Mixed anhydrides are synthesized in high yield and isolated by paper or DEAE-cellulose column chromatography. The ability of such anhydrides to phosphorylate different nucleophilic agents was used for synthesis of amidates, imidazolidates, esters, thioesters and pyrophosphates of mono- and oligonucleotides. Mixed anhydrides mono-, oligonucleotides and nucleosidedi-(tri)phosphates and mesitoic acid were also applied to achieve immobilization of the mono- and oligonucleotides via their end groups on hexamethylenediamine - Sepharose support. Mixed anhydrides studied may be efficiently used for affinity labeling of proteins and nucleic acids and also as material for preparating reagents for template reactions.
描述了一种通过与均苯三甲酸形成混合酸酐来选择性激活寡核苷酸和核苷二（三）磷酸的磷酸单酯端基的方法。混合酸酐以高产率合成，并通过纸色谱或DEAE-纤维素柱色谱分离。此类酸酐使不同亲核试剂磷酸化的能力被用于合成单核苷酸和寡核苷酸的酰胺、咪唑啉酸酯、酯、硫酯和焦磷酸酯。单核苷酸、寡核苷酸和核苷二（三）磷酸与均苯三甲酸形成的混合酸酐还被用于通过其端基将单核苷酸和寡核苷酸固定在六亚甲基二胺-琼脂糖载体上。所研究的混合酸酐可有效地用于蛋白质和核酸的亲和标记，也可作为制备模板反应试剂的材料。
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