抗炎药物对钙在各种人体和豚鼠组织细胞膜上结合的影响。

Effect of anti-inflammatory drugs on the binding of calcium to cellular membranes in various human and guinea-pig tissues.

Northover B J

Br J Pharmacol. 1973 Jul;48(3):496-504. doi: 10.1111/j.1476-5381.1973.tb08356.x.

Abstract

Cellular membranes were obtained by centrifugation of homogenates of various human and guinea-pig tissues. These membranes took up added calcium (Ca) when incubated at 25 degrees C for 10 min in histidine buffer at pH 7.4. The uptake of Ca was increased by the presence of adenosine triphosphate (ATP).2. Evidence is presented that the membrane fragments which took up Ca under these conditions were derived from the endoplasmic reticulum.3. The ATP-dependent uptake of Ca by membranes derived from guinea-pig aortic or gastric smooth muscle or from human umbilical arterial muscle was inhibited by indomethacin in a concentration-dependent manner.4. The ATP-dependent uptake of Ca by membranes derived from heart, brain, skeletal muscle or liver of the guinea-pig was not inhibited by indomethacin.5. Endothelial cells were obtained from the lining of the human umbilical vein. Cellular membranes were prepared from the endothelial cells by homogenization and centrifugation. The endothelial membranes took up added Ca in the presence of added ATP and this uptake was inhibited by indomethacin and also by members of a group of pharmacologically-related drugs.

摘要

通过对各种人类和豚鼠组织匀浆进行离心获得细胞膜。这些细胞膜在pH 7.4的组氨酸缓冲液中于25℃孵育10分钟时会摄取添加的钙（Ca）。三磷酸腺苷（ATP）的存在会增加钙的摄取。
有证据表明，在这些条件下摄取钙的膜片段源自内质网。
吲哚美辛以浓度依赖的方式抑制豚鼠主动脉或胃平滑肌或人脐动脉肌来源的膜对ATP依赖的钙摄取。
吲哚美辛不抑制豚鼠心脏、脑、骨骼肌或肝脏来源的膜对ATP依赖的钙摄取。
从人脐静脉内膜获得内皮细胞。通过匀浆和离心从内皮细胞制备细胞膜。内皮细胞膜在添加ATP的情况下摄取添加的钙，这种摄取受到吲哚美辛以及一组药理相关药物成员的抑制。