Moritoki H, Takei M, Kasai T, Matsumura Y, Ishida Y
J Pharmacol Exp Ther. 1979 Oct;211(1):104-11.
ATP and other adenine derivatives, such as AMP and adenosine, at concentrations above 10(-6) M induced dose-dependent contractions of guinea-pig uterine strips. Treatment of the strips with nonsteroidal anti-inflammatory drugs, such as indomethacin, aspirin and phenylbutazone, at concentrations of 10(-6) to 10(-4) M irreversibly inhibited the contractions, without affecting those caused by acetylcholine and bradykinin. Arachidonic acid (10(-8)-10(-6) g/ml) and prostaglandins (E1, E2 and F2 alpha, 10(-9)-10(-7) g/ml) restored the inhibited uterine response to ATP, but the inhibition was reinstated on washing out of the arachidonic acid or prostaglandins. Furthermore, the prostaglandin antagonists polyphloretin phosphate (3 x 10(-5)-3 x 10(-4) g/ml) and SC 19220 (10(-6)-3 X 10(-5) M) selectively suppressed the action of ATP. In addition to the prostaglandin antagonists, 2,2'-pyridylisatogen, reported to be an ATP antagonist, at concentrations of 10(-6) to 3 x 10(-5) M selectively inhibited the response of uterine strips to ATP. These results suggest the involvement of prostaglandins in the actions of ATP and other adenine derivatives on guinea-pig uterine tissue and provide further evidence for ATP-stimulated prostaglandin formation in smooth muscle.
ATP及其他腺嘌呤衍生物，如AMP和腺苷，浓度高于10⁻⁶ M时可引起豚鼠子宫条剂量依赖性收缩。用非甾体抗炎药，如吲哚美辛、阿司匹林和保泰松，浓度为10⁻⁶至10⁻⁴ M处理子宫条，可不可逆地抑制收缩，而不影响乙酰胆碱和缓激肽引起的收缩。花生四烯酸（10⁻⁸ - 10⁻⁶ g/ml）和前列腺素（E1、E2和F2α，10⁻⁹ - 10⁻⁷ g/ml）可恢复子宫对ATP的抑制反应，但洗去花生四烯酸或前列腺素后抑制作用又恢复。此外，前列腺素拮抗剂多聚磷酸根皮苷（3×10⁻⁵ - 3×10⁻⁴ g/ml）和SC 19220（10⁻⁶ - 3×10⁻⁵ M）可选择性抑制ATP的作用。除了前列腺素拮抗剂外，据报道为ATP拮抗剂的2,2'-吡啶异吲哚原，浓度为10⁻⁶至3×10⁻⁵ M时可选择性抑制子宫条对ATP的反应。这些结果表明前列腺素参与了ATP及其他腺嘌呤衍生物对豚鼠子宫组织的作用，并为平滑肌中ATP刺激前列腺素形成提供了进一步证据。
