高氯酸钠在核酸制备过程中用于脱蛋白的应用。
The use of sodium perchlorate in deproteinization during the preparation of nucleic acids.
作者信息
Wilcockson J
出版信息
Biochem J. 1973 Nov;135(3):559-61. doi: 10.1042/bj1350559.
Abstract
Sodium perchlorate in high concentrations will remove from solution the detergent sodium dodecyl sulphate and protein complexed with it. This and the failure of proteins to be precipitated by ethanol from solutions containing a high concentration of sodium perchlorate can be utilized as efficient, rapid and simple deproteinization procedures during the preparation of nucleic acids.
摘要
高浓度的高氯酸钠会从溶液中去除洗涤剂十二烷基硫酸钠及其与之结合的蛋白质。在核酸制备过程中，高氯酸钠的这种作用以及蛋白质在含有高浓度高氯酸钠的溶液中不能被乙醇沉淀的特性，可被用作高效、快速且简单的脱蛋白程序。
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