禽成髓细胞瘤病毒包膜蛋白的酶促放射性碘化
Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus.
作者信息
Fritz R B
出版信息
J Virol. 1974 Jan;13(1):42-5. doi: 10.1128/JVI.13.1.42-45.1974.
Abstract
Purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. Disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. Reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this technique to be useful for the isolation of viral envelope antigens.
摘要
用乳过氧化物酶法对纯化的禽成髓细胞瘤病毒进行碘化。对标记病毒粒子的破坏以及病毒蛋白的色谱分析表明，放射性碘仅与病毒包膜的糖蛋白相关。将放射性标记的病毒与抗病毒抗体反应，随后用温和去污剂处理并回收免疫复合物，结果表明该技术可用于分离病毒包膜抗原。
相似文献
1
Enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus.禽成髓细胞瘤病毒包膜蛋白的酶促放射性碘化
J Virol. 1974 Jan;13(1):42-5. doi: 10.1128/JVI.13.1.42-45.1974.
2
Localization of RNA tumor virus polypeptides. I. Isolation of further virus substructures.RNA肿瘤病毒多肽的定位。I. 进一步病毒亚结构的分离。
Virology. 1973 Dec;56(2):549-64. doi: 10.1016/0042-6822(73)90057-3.
3
Polypeptides of avian RNA tumor viruses. IV. Components of the viral envelope.禽RNA肿瘤病毒的多肽。IV.病毒包膜的成分。
Virology. 1972 Mar;47(3):551-66. doi: 10.1016/0042-6822(72)90545-4.
4
Outer membrane of avian myeloblastosis virus.禽成髓细胞瘤病毒外膜
J Virol. 1973 Dec;12(6):1579-88. doi: 10.1128/JVI.12.6.1579-1588.1973.
5
Radioimmunoassay of avian RNA tumor virus group-specific antigen.禽RNA肿瘤病毒群特异性抗原的放射免疫测定
Intervirology. 1973;1(2):119-26. doi: 10.1159/000148838.
6
Isolation and chemical characterization of the major envelope glycoprotein of avian myeloblastosis virus.禽成髓细胞瘤病毒主要包膜糖蛋白的分离与化学特性分析
J Biol Chem. 1980 Feb 25;255(4):1277-83.
7
Structural studies of avian myeloblastosis virus: comparison of polypeptides in virion and core component by dodecyl sulfate-polyacrylamide gel electrophoresis.禽成髓细胞瘤病毒的结构研究：通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳比较病毒粒子和核心成分中的多肽
J Virol. 1974 Feb;13(2):513-28. doi: 10.1128/JVI.13.2.513-528.1974.
8
Isolation of a second avian leukosis group-specific antigen (gs-b) from avian myeloblastosis virus.
Proc Natl Acad Sci U S A. 1970 Oct;67(2):837-42. doi: 10.1073/pnas.67.2.837.
9
Structural components of avian myeloblastosis virus.
Bibl Haematol. 1973;39:316-30. doi: 10.1159/000427858.
10
Isolation of the major envelope glycoprotein of avian myeloblastosis virus.禽成髓细胞瘤病毒主要包膜糖蛋白的分离
Methods Enzymol. 1982;83:320-6. doi: 10.1016/0076-6879(82)83027-9.
引用本文的文献
1
In vitro radioisotopic labeling of proteins associated with purified polyoma virions.与纯化的多瘤病毒粒子相关的蛋白质的体外放射性同位素标记
J Virol. 1974 Dec;14(6):1627-9. doi: 10.1128/JVI.14.6.1627-1629.1974.
2
Structure of the mouse mammary tumor virus: characterization of bald particles.小鼠乳腺肿瘤病毒的结构：光秃颗粒的特征
J Virol. 1974 Nov;14(5):1293-303. doi: 10.1128/JVI.14.5.1293-1303.1974.
3
In vitro radiolabeling procedure which labels the proteins of Newcastle disease virions with carbon-14.用碳-14对新城疫病毒粒子的蛋白质进行标记的体外放射性标记程序。
Infect Immun. 1975 Dec;12(6):1411-4. doi: 10.1128/iai.12.6.1411-1414.1975.
4
Proteins of Epstein-Barr Virus. II. Electrophoretic analysis of the polypeptides of the nucleocapsid and the glucosamine- and polysaccharide-containing components of enveloped virus.
J Virol. 1976 Apr;18(1):289-97. doi: 10.1128/JVI.18.1.289-297.1976.
本文引用的文献
1
Radioiodination of antibodies and adsorbed to insoluble antigens.抗体的放射性碘化及吸附于不溶性抗原。
J Immunol. 1967 Jan;98(1):67-71.
2
The arrangement of proteins in the human erythrocyte membrane.人类红细胞膜中蛋白质的排列
Biochem Biophys Res Commun. 1970 Jul 27;40(2):284-9. doi: 10.1016/0006-291x(70)91007-7.
3
The polypeptides of influenza virus. V. Localization of polypeptides in the virion by iodination techniques.流感病毒的多肽。V. 用碘化技术对病毒粒子中多肽的定位
Virology. 1971 Dec;46(3):764-73. doi: 10.1016/0042-6822(71)90078-x.
4
The enzymatic iodination of the red cell membrane.红细胞膜的酶促碘化作用。
J Cell Biol. 1972 Nov;55(2):390-405. doi: 10.1083/jcb.55.2.390.
5
Enzymatic iodination of Sindbis virus proteins.辛德毕斯病毒蛋白的酶促碘化作用。
J Virol. 1973 May;11(5):730-5. doi: 10.1128/JVI.11.5.730-735.1973.
6
Synthesis, biochemistry and dynamics of cell surface immunoglobulin on lymphocytes.淋巴细胞表面免疫球蛋白的合成、生物化学及动力学
Fed Proc. 1973 Jan;32(1):35-40.
7
Isolation of theta antigen from the surface of thymus lymphocytes.从胸腺淋巴细胞表面分离θ抗原。
Nat New Biol. 1973 Feb 21;241(112):251-2. doi: 10.1038/newbio241251a0.
8
Identification of a large polypeptide precursor of avian oncornavirus proteins.禽肿瘤病毒蛋白大多肽前体的鉴定
Proc Natl Acad Sci U S A. 1973 Jun;70(6):1734-8. doi: 10.1073/pnas.70.6.1734.
9
Avian oncornavirus group-specific antigen: detection and quantification by radioimmunoassay.禽肿瘤病毒群特异性抗原：放射免疫测定法进行检测与定量
J Virol. 1973 May;11(5):736-40. doi: 10.1128/JVI.11.5.736-740.1973.
10
Chromatographic separation and antigenic analysis of proteins of the oncornaviruses. I. Avian leukemia-sarcoma viruses.肿瘤病毒蛋白质的色谱分离与抗原分析。I. 禽白血病-肉瘤病毒
J Virol. 1971 Nov;8(5):778-85. doi: 10.1128/JVI.8.5.778-785.1971.
Zotero 插件