与纯化的多瘤病毒粒子相关的蛋白质的体外放射性同位素标记
In vitro radioisotopic labeling of proteins associated with purified polyoma virions.
作者信息
McMillen J, Consigli R A
出版信息
J Virol. 1974 Dec;14(6):1627-9. doi: 10.1128/JVI.14.6.1627-1629.1974.
Abstract
An in vitro procedure using [(14)C]formaldehyde and sodium borohydride was used to label the proteins of purified polyoma virions. This method, which labeled the three capsid proteins and the four internal histones of polyoma virus, did not alter the biological and biophysical characteristics of the virus. In addition to the seven viral proteins, five additional radioactive peaks were resolved on polyacrylamide gels. Four of these peaks could be attributed to growing the virus in the presence of serum. The fifth peak appears to be a nonhistone host-contributed protein made before infection.
摘要
使用[(14)C]甲醛和硼氢化钠的体外程序来标记纯化的多瘤病毒颗粒的蛋白质。该方法标记了多瘤病毒的三种衣壳蛋白和四种内部组蛋白，并未改变病毒的生物学和生物物理特性。除了这七种病毒蛋白外，在聚丙烯酰胺凝胶上还分辨出另外五个放射性峰。其中四个峰可归因于在血清存在下培养病毒。第五个峰似乎是感染前由宿主贡献的一种非组蛋白。
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