一种用于收集和检测放射性大分子梯度级分的快速方法。
A rapid method for collecting and assaying gradient fractions for radioactive macromolecules.
作者信息
Rubinstein L, Rein A
出版信息
Biochem J. 1974 Jan;137(1):131-3. doi: 10.1042/bj1370131.
Abstract
A rapid method is described for collecting gradients after centrifugation, and assaying them for acid-precipitable radioactivity. An entire gradient is collected and processed on a single glass-fibre filter disc. This method allows one easily to assay six gradients for acid-precipitable radioactivity in less than 2h.
摘要
本文描述了一种用于在离心后收集梯度并测定其酸沉淀放射性的快速方法。整个梯度在单个玻璃纤维滤盘上收集和处理。该方法可让人们在不到2小时的时间内轻松测定六个梯度的酸沉淀放射性。
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A rapid method for collecting and assaying gradient fractions for radioactive macromolecules.一种用于收集和检测放射性大分子梯度级分的快速方法。
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本文引用的文献
1
Band-centrifugation of macromolecules and viruses in self-generating density gradients.在自生成密度梯度中对大分子和病毒进行区带离心。
Proc Natl Acad Sci U S A. 1963 Jun;49(6):902-10. doi: 10.1073/pnas.49.6.902.
2
Thermal conversion of nonpriming deoxyribonucleic acid to primer.非引发脱氧核糖核酸向引物的热转化
J Biol Chem. 1959 Oct;234:2733-4.
3
Reconstruction in vivo of irradiated Escherichia coli deoxyribonucleic acid; the rejoining of broken pieces.受辐照大肠杆菌脱氧核糖核酸的体内重建；断裂片段的重新连接。
Nature. 1966 Oct 29;212(5061):534-5. doi: 10.1038/212534a0.
4
A very rapid method for washing large numbers of precipitates of proteins and nucleic acids.一种用于洗涤大量蛋白质和核酸沉淀物的非常快速的方法。
Anal Biochem. 1971 Oct;43(2):639-40. doi: 10.1016/0003-2697(71)90300-9.
5
Paper strip method for assaying gradient fractions containing radioactive macromolecules.用于检测含有放射性大分子的梯度级分的纸条法。
Anal Biochem. 1971 Oct;43(2):427-32. doi: 10.1016/0003-2697(71)90272-7.
6
Selective extraction of polyoma DNA from infected mouse cell cultures.从受感染的小鼠细胞培养物中选择性提取多瘤病毒DNA。
J Mol Biol. 1967 Jun 14;26(2):365-9. doi: 10.1016/0022-2836(67)90307-5.
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