猴肾细胞中猿猴病毒40复制DNA的定位
Localization of replicating DNA of simian virus 40 in monkey kidney cells.
作者信息
LeBlanc D J, Singer M F
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2236-40. doi: 10.1073/pnas.71.6.2236.
Abstract
Approximately 80-85% of the DNA synthesized by cells infected with simian virus 40 during a short pulse with [(3)H]thymidine is associated with host nuclear membrane components, as defined by the M-band technique. A substantial portion of this membrane-associated DNA has been shown to be viral specific by sedimentation studies and by DNA.DNA hybridization. Examination of DNA associated and unassociated with nuclear M-band by neutral sucrose gradient analysis suggested that while replicating DNA of simian virus 40 is attached to the nuclear membrane, mature component I DNA of simian virus 40 is released from the membrane upon completion of replication.
摘要
用[³H]胸腺嘧啶短时间脉冲处理感染猿猴病毒40的细胞后，通过M带技术定义，约80 - 85%合成的DNA与宿主核膜成分相关。沉降研究和DNA-DNA杂交已表明，这部分与膜相关的DNA很大一部分是病毒特异性的。通过中性蔗糖梯度分析检测与核M带相关和不相关的DNA表明，虽然猿猴病毒40的复制型DNA附着在核膜上，但猿猴病毒40成熟的I型成分DNA在复制完成后从膜上释放。
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