Schimmel R J
J Lipid Res. 1974 May;15(3):206-10.
Segments of epididymal adipose tissue from normal male rats were incubated with micromolar concentrations of colchicine for different periods of time up to 4 hr, and the mobilization of free fatty acids (FFA) was measured during a subsequent reincubation. Although pretreatment with colchicine did not alter basal unstimulated FFA release, mobilization of FFA in the presence of epinephrine or theophylline was reduced. However, neither lipolysis, as judged by glycerol production, nor cyclic AMP accumulation was impaired under the same conditions. To assess the possibility that colchicine might limit production of fatty acids by accelerating the entry and metabolism of glucose into adipocytes, the metabolism of glucose by adipose tissue was studied. Pretreatment with colchicine did not affect uptake of glucose nor its oxidation to CO(2), although colchicine-treated tissues did have slightly more [(14)C]glucose incorporated into the glyceride moiety of triglyceride. When adipose tissues pretreated with colchicine were incubated in an albumin-free medium, no reduction in FFA production by colchicine was observed. Because no FFA release occurs in albumin-free media, this experiment suggests that colchicine-induced inhibition of FFA mobilization results from impaired extrusion of FFA from adipose cells.
将正常雄性大鼠附睾脂肪组织的片段与微摩尔浓度的秋水仙碱孵育不同时间，最长达4小时，然后在随后的再孵育过程中测量游离脂肪酸（FFA）的动员情况。尽管用秋水仙碱预处理并未改变基础状态下未受刺激的FFA释放，但在肾上腺素或茶碱存在的情况下，FFA的动员减少。然而，在相同条件下，无论是通过甘油生成判断的脂解作用，还是环磷酸腺苷（cAMP）的积累均未受损。为了评估秋水仙碱是否可能通过加速葡萄糖进入脂肪细胞并进行代谢来限制脂肪酸的产生，对脂肪组织的葡萄糖代谢进行了研究。秋水仙碱预处理并未影响葡萄糖的摄取及其氧化为CO₂，尽管用秋水仙碱处理的组织确实有稍多的[¹⁴C]葡萄糖掺入甘油三酯的甘油部分。当将用秋水仙碱预处理的脂肪组织在无白蛋白培养基中孵育时，未观察到秋水仙碱对FFA产生的减少作用。由于在无白蛋白培养基中不会发生FFA释放，该实验表明秋水仙碱诱导的FFA动员抑制是由于脂肪细胞中FFA的挤出受损所致。
