Sugita M, Iwamori M, Evans J, McCluer R H, Dulaney J T, Moser H W
J Lipid Res. 1974 May;15(3):223-6.
Conditions have been determined for the benzoylation of ceramides containing nonhydroxy and hydroxy fatty acids, and a high performance liquid chromatography system for the separation and measurement of these derivatives has been devised that is capable of good resolution and high sensitivity. These methods have been used to determine quantitatively the levels of ceramides in human tissues, and in serum and urine, and to demonstrate elevated amounts of ceramide in Farber's disease urine and tissues.
已确定含有非羟基和羟基脂肪酸的神经酰胺进行苯甲酰化的条件，并设计了一种用于分离和测量这些衍生物的高效液相色谱系统，该系统具有良好的分辨率和高灵敏度。这些方法已用于定量测定人体组织、血清和尿液中的神经酰胺水平，并证明法伯病患者尿液和组织中的神经酰胺含量升高。
