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甲状腺激素作用：在分离的细胞核和可溶性核提取物中对假定受体的体外证明。

Thyroid hormone action: in vitro demonstration of putative receptors in isolated nuclei and soluble nuclear extracts.

作者信息

Samuels H H, Tsai J S, Casanova J

出版信息

Science. 1974 Jun 14;184(4142):1188-91. doi: 10.1126/science.184.4142.1188.

DOI:10.1126/science.184.4142.1188
PMID:4364362
Abstract

Saturable binding activities for triiodothyronine were demonstrated in vitro with isolated nuclei and soluble nuclear extracts of rat liver, kidney, and cultured GH(1) cells. The binding activity can be extracted from nuclei in soluble form with no significant change in hormone affinity and has properties of a nonhistone protein.

摘要

在体外，用大鼠肝脏、肾脏和培养的GH(1)细胞的分离细胞核及可溶性核提取物证实了三碘甲状腺原氨酸的饱和结合活性。该结合活性可以以可溶形式从细胞核中提取出来，激素亲和力无显著变化，并且具有非组蛋白的特性。

相似文献

1
Thyroid hormone action: in vitro demonstration of putative receptors in isolated nuclei and soluble nuclear extracts.甲状腺激素作用：在分离的细胞核和可溶性核提取物中对假定受体的体外证明。
Science. 1974 Jun 14;184(4142):1188-91. doi: 10.1126/science.184.4142.1188.
2
Thyroid hormone action: in vitro characterization of solubilized nuclear receptors from rat liver and cultured GH1 cells.甲状腺激素作用：大鼠肝脏和培养的GH1细胞中可溶性核受体的体外特性研究
J Clin Invest. 1974 Oct;54(4):853-65. doi: 10.1172/JCI107825.
3
[Triiodothyronine binding by the liver nuclei and mitochondria].[肝脏细胞核和线粒体对三碘甲状腺原氨酸的结合]
Probl Endokrinol (Mosk). 1979 Nov-Dec;25(6):60-5.
4
Thyroid hormone action in cell culture: domonstration of nuclear receptors in intact cells and isolated nuclei.甲状腺激素在细胞培养中的作用：完整细胞和分离细胞核中核受体的证明。
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3488-92. doi: 10.1073/pnas.70.12.3488.
5
In vitro binding of L-triiodothyronine to receptors in rat liver nuclei. Kinectics of binding, extraction properties, and lack of requirement for cytosol proteins.L-三碘甲状腺原氨酸与大鼠肝细胞核中受体的体外结合。结合动力学、提取特性以及对胞质溶胶蛋白的需求缺失。
J Clin Invest. 1975 Jan;55(1):50-60. doi: 10.1172/JCI107917.
6
In vitro 3,5,3'-triiodothyronine binding to rat cerebrocortical neuronal and glial nuclei suggests the presence of binding sites unavailable in vivo.体外实验中，3,5,3'-三碘甲状腺原氨酸与大鼠大脑皮质神经元及神经胶质细胞核的结合表明，存在体内无法利用的结合位点。
Endocrinology. 1985 May;116(5):2019-28. doi: 10.1210/endo-116-5-2019.
7
Thyroid hormone action. Demonstration of similar receptors in isolated nuclei of rat liver and cultured GH1 cells.甲状腺激素作用。在大鼠肝脏分离细胞核和培养的GH1细胞中证明存在相似受体。
J Clin Invest. 1974 Feb;53(2):656-9. doi: 10.1172/JCI107601.
8
Modulation of nuclear receptor affinity for 3,5,3'-triiodothyronine by nucleotide in vitro.
Endocrinol Exp. 1985 Dec;19(4):245-51.
9
Thyroid hormone action: demonstration of putative nuclear receptors in human lymphocytes.甲状腺激素作用：人类淋巴细胞中假定核受体的证明。
J Clin Endocrinol Metab. 1974 May;38(5):919-22. doi: 10.1210/jcem-38-5-919.
10
Evidence for an active step in thyroid hormone transport to nuclei: drug inhibition of L-125I-triiodothyronine binding to nuclear receptors in rat pituitary tumor cells.甲状腺激素向细胞核转运过程中存在活跃步骤的证据：药物对大鼠垂体肿瘤细胞中L-125I-三碘甲状腺原氨酸与核受体结合的抑制作用。
Endocrinology. 1982 Mar;110(3):1070-2. doi: 10.1210/endo-110-3-1070.

引用本文的文献

1
Nuclear hormone receptor functions in keratinocyte and melanocyte homeostasis, epidermal carcinogenesis and melanomagenesis.核激素受体在角质形成细胞和黑素细胞的稳态、表皮癌发生和黑色素瘤发生中发挥作用。
FEBS Lett. 2013 Mar 18;587(6):529-41. doi: 10.1016/j.febslet.2013.01.041. Epub 2013 Feb 5.
2
Identification of differentially expressed thyroid hormone responsive genes from the brain of the Mexican Axolotl (Ambystoma mexicanum).从墨西哥钝口螈（Ambystoma mexicanum）脑中鉴定甲状腺激素反应差异表达基因。
Comp Biochem Physiol C Toxicol Pharmacol. 2012 Jan;155(1):128-35. doi: 10.1016/j.cbpc.2011.03.006. Epub 2011 Mar 30.
3
Endogenous ligands for nuclear receptors: digging deeper.
核受体的内源性配体：深挖。
J Biol Chem. 2010 Dec 24;285(52):40409-15. doi: 10.1074/jbc.R110.182451. Epub 2010 Oct 18.
4
Molecular aspects of thyroid hormone actions.甲状腺激素作用的分子方面。
Endocr Rev. 2010 Apr;31(2):139-70. doi: 10.1210/er.2009-0007. Epub 2010 Jan 5.
5
Thyroid hormone action: in vitro characterization of solubilized nuclear receptors from rat liver and cultured GH1 cells.甲状腺激素作用：大鼠肝脏和培养的GH1细胞中可溶性核受体的体外特性研究
J Clin Invest. 1974 Oct;54(4):853-65. doi: 10.1172/JCI107825.
6
Contributions of plasma triiodothyronine and local thyroxine monodeiodination to triiodothyronine to nuclear triiodothyronine receptor saturation in pituitary, liver, and kidney of hypothyroid rats. Further evidence relating saturation of pituitary nuclear triiodothyronine receptors and the acute inhibition of thyroid-stimulating hormone release.甲状腺功能减退大鼠垂体、肝脏和肾脏中血浆三碘甲状腺原氨酸及局部甲状腺素单碘脱碘作用对三碘甲状腺原氨酸与核三碘甲状腺原氨酸受体饱和的贡献。关于垂体细胞核三碘甲状腺原氨酸受体饱和与促甲状腺激素释放急性抑制之间关系的进一步证据。
J Clin Invest. 1978 May;61(5):1247-59. doi: 10.1172/JCI109041.
7
Thyroid hormone binding by a component of mitochondrial membrane.甲状腺激素与线粒体膜的一种成分结合。
Proc Natl Acad Sci U S A. 1975 Aug;72(8):3225-9. doi: 10.1073/pnas.72.8.3225.