肝脏中葡萄糖抑制过程中磷酸烯醇式丙酮酸羧激酶可翻译信使核糖核酸的快速丧失。
Rapid loss of translatable messenger RNA of phosphoenolpyruvate carboxykinase during glucose repression in liver.
作者信息
Tilghman S M, Hanson R W, Reshef L, Hopgood M F, Ballard F J
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1304-8. doi: 10.1073/pnas.71.4.1304.
The rate of synthesis of phosphoenolpyruvate carboxykinase (EC 4.1.1.32) in rat liver decreased with a half-life of 30 min after fasted rats were refed either a chow diet or glucose. A requirement for both glucose and insulin to bring about this rapid deinduction was shown, as well as the ability of dibutyryl adenosine 3':5'-cyclic monophosphate to block the decrease in enzyme synthesis. Estimates of the stability of messenger RNA of phosphoenolpyruvate carboxykinase were made by using the inhibitors actinomycin D and cordycepin to block further messenger RNA synthesis, and then measuring the decrease in specific enzyme synthesis. It is suggested that the use of actinomycin D yields an overestimation of the template stability. The results with cordycepin imply that the enzyme messenger RNA has a short half-life of approximately 1 hr. Thus, it is possible that deinduction may proceed by way of a decrease in messenger RNA production, leading to a rapid fall in the synthesis of phosphoenolpyruvate carboxykinase.
给禁食的大鼠重新喂食普通饲料或葡萄糖后，大鼠肝脏中磷酸烯醇式丙酮酸羧激酶（EC 4.1.1.32）的合成速率下降，半衰期为30分钟。研究表明，葡萄糖和胰岛素对这种快速去诱导都是必需的，同时二丁酰腺苷3':5'-环磷酸也有阻断酶合成下降的能力。通过使用放线菌素D和虫草素这两种抑制剂来阻断进一步的信使核糖核酸合成，然后测量特定酶合成的下降情况，对磷酸烯醇式丙酮酸羧激酶信使核糖核酸的稳定性进行了估计。结果表明，使用放线菌素D会高估模板稳定性。虫草素的实验结果表明，该酶的信使核糖核酸半衰期较短，约为1小时。因此，去诱导可能是通过信使核糖核酸产生减少来进行的，从而导致磷酸烯醇式丙酮酸羧激酶的合成迅速下降。
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