用电子显微镜观察RNA肿瘤病毒的单链核酸。
Visualization of single-stranded nucleic acid of RNA tumor virus with the electron microscope.
作者信息
Weber G H, Heine U, Cottler-Fox M, Beaudreau G S
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1887-90. doi: 10.1073/pnas.71.5.1887.
Abstract
60-70S RNA of avian myeloblastosis virus was spread with the Kleinschmidt method and examined in the electron microscope after being subjected to a variety of denaturing conditions. Extension of the molecules from a collapsed configuration was seen only under stringent denaturing conditions, resulting in molecules of 1-2 mum in length. Longer strands of these extremely fragile molecules cannot be obtained with the methods presently available.
摘要
禽成髓细胞瘤病毒的60 - 70S RNA采用克莱因施密特方法铺展，并在经过各种变性条件处理后用电子显微镜检查。只有在严格的变性条件下才能看到分子从折叠构象伸展，形成长度为1 - 2微米的分子。用目前可用的方法无法获得这些极其脆弱分子的更长链。
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