禽成髓细胞瘤病毒RNA序列的体外合成
The in vitro synthesis of avian myeloblastosis viral RNA sequences.
作者信息
Jacquet M, Groner Y, Monroy G, Hurwitz J
出版信息
Proc Natl Acad Sci U S A. 1974 Aug;71(8):3045-9. doi: 10.1073/pnas.71.8.3045.
Abstract
Isolated nuclei, prepared from myeloblasts of chicks infected with avian myeloblastosis virus, synthesize RNA sequences present in avian myeloblastosis viral RNA. These sequences are also formed during transcription of chromatin, isolated from myeloblasts, by DNA-dependent RNA polymerases purified from Escherichia coli or calfthymus. In the latter case, transcription is alpha-amanitin sensitive. Formation of hybrids between RNA and avian myeloblastosis virus DNA probes has been monitored by the combined use of ribonucleases A, T(1), and H, and ribonucleases specific for single strands.
摘要
从感染禽成髓细胞瘤病毒的雏鸡成髓细胞中制备的分离细胞核，能合成禽成髓细胞瘤病毒RNA中存在的RNA序列。这些序列也在从成髓细胞分离的染色质转录过程中形成，该转录由从大肠杆菌或小牛胸腺中纯化的依赖DNA的RNA聚合酶进行。在后一种情况下，转录对α-鹅膏蕈碱敏感。通过联合使用核糖核酸酶A、T(1)和H以及单链特异性核糖核酸酶，监测了RNA与禽成髓细胞瘤病毒DNA探针之间杂交体的形成。
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