Repine J E, White J G, Clawson C C, Holmes B M
J Clin Invest. 1974 Jul;54(1):83-90. doi: 10.1172/JCI107752.
Previous investigations have demonstrated that phorbol myristate acetate (PMA), the active principle of croton oil, stimulates alterations in normal polymorphonuclear leukocytes (PMN) that resemble closely the changes that develop in the cells after phagocytosis of bacteria. The present study has compared the effects of PMA and heat-killed bacteria on the oxygen uptake, glucose oxidation, nitroblue tetrazolium (NBT) reduction, and ultrastructure of normal neutrophils and PMN from six patients with chronic granulomatous disease (CGD). PMA stimulated oxygen consumption, hexose monophosphate shunt activity, and NBT reduction in normal cells but failed to produce similar effects in CGD neutrophils. However, PMA did induce formation of cytoplasmic vacuoles in the CGD cells similar to those observed in normal neutrophils. The results indicate that PMA is a useful nonparticulate agent for distinguishing between normal and CGD neutrophils and for studying basic mechanisms of phagocytosis in normal and abnormal PMN.
以往的研究表明，巴豆油的活性成分佛波酯（PMA）可刺激正常多形核白细胞（PMN）发生改变，这些改变与细菌吞噬后细胞中出现的变化极为相似。本研究比较了PMA和热灭活细菌对6例慢性肉芽肿病（CGD）患者的正常中性粒细胞和PMN的氧摄取、葡萄糖氧化、硝基蓝四氮唑（NBT）还原及超微结构的影响。PMA可刺激正常细胞的氧消耗、磷酸己糖旁路活性和NBT还原，但对CGD中性粒细胞未产生类似作用。然而，PMA确实可诱导CGD细胞形成胞质空泡，类似于在正常中性粒细胞中观察到的空泡。结果表明，PMA是一种有用的非颗粒性试剂，可用于区分正常和CGD中性粒细胞，以及研究正常和异常PMN的吞噬基本机制。
