COHN Z A, MORSE S I
J Exp Med. 1960 May 1;111(5):689-704. doi: 10.1084/jem.111.5.689.
The effects of a purified bacterial lipopolysaccharide endotoxin on homogenous populations of rabbit polymorphonuclear leucocytes have been studied in vitro under defined conditions. Employing a 500-fold range of concentration (0.1 to 50.0 microg./ml.), it was shown that endotoxin enhanced the rate at which staphylococci were killed by leucocytes. The mechanism underlying the increased killing was found to be a direct stimulation of the phagocytic activity of the leucocyte and not mediated by the release of bactericidins or opsonins from the treated cells. In the presence of 10 per cent serum all concentrations of endotoxin enhanced phagocytosis, whereas at lower serum concentrations, the higher doses of lipopolysaccharide inhibited the phagocytic activity of the cells. Similar concentrations of endotoxin were capable of increasing the utilization of glucose and the production of lactic acid. Endotoxin treated leucocytes exhibited no change in oxygen consumption, and only a slight depression in glycogen synthesis. It appeared that endotoxin could interact and alter the functional and metabolic properties of leucocytes in the absence of serum. The demonstration of enhanced phagocytic activity of endotoxin-treated cells was dependent upon the particular opsonic requirements for the organism under study.
在特定条件下，对纯化的细菌脂多糖内毒素作用于家兔多形核白细胞同质性群体的效应进行了体外研究。采用500倍浓度范围（0.1至50.0微克/毫升），结果表明内毒素提高了白细胞杀灭葡萄球菌的速率。发现杀灭作用增强的潜在机制是对白细胞吞噬活性的直接刺激，而非由处理过的细胞释放杀菌素或调理素介导。在10%血清存在的情况下，所有浓度的内毒素均增强吞噬作用，而在较低血清浓度时，较高剂量的脂多糖会抑制细胞的吞噬活性。相似浓度的内毒素能够增加葡萄糖的利用和乳酸的产生。经内毒素处理的白细胞耗氧量无变化，糖原合成仅有轻微降低。看来内毒素在无血清情况下能够相互作用并改变白细胞的功能和代谢特性。内毒素处理细胞吞噬活性增强的证明取决于所研究生物体的特定调理需求。
